Anticorps Monoclonal anti-Foxp3

Foxp3 Monoclonal Antibody for FC (Intra)

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

souris

Applications

FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

Publications(1)

CloneNo.

3G3

N° de cat : CL488-65089

Synonymes

forkhead box P3, Foxp3, JM2, scurfin, sf

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Galerie de données de validation

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  FC experiment of mouse splenocytes using CL488-65089

  1x10^6 mouse splenocytes were intracellularly stained with 0.06 ug CoraLite® Plus 488 Anti-Mouse Foxp3 (CL488-65089, Clone:3G3), and 0.06 ug CoraLite® Plus 647 Anti-Mouse CD4 (GK1.5) (CL647-65104, Clone: GK1.5), and 0.06 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en FC (Intra): splénocytes de souris
• Résultats positifs en cytométrie: splénocytes de souris

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-65089 cible Foxp3 dans les applications de FC (Intra) et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: souris
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: s/o
• Nom complet: forkhead box P3
• Numéro d’acquisition GenBank: BC132333
• Symbole du gène: Foxp3
• Identification du gène (NCBI): 20371
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Foxp3 (forkhead box P3), also named as IPEX, JM2 and scurfin, is a member of the forkhead transcription factor family and is essential for normal immune homeostasis (PMID: 11138001; 20429413). Foxp3 is specifically expressed in CD4+ CD25+ regulatory T cells and is crucial for their development and suppressive activity (PMID: 12612578; 12612581). In scurfy mouse, disruption of Foxp3 results in the fatal lymphoproliferative disorder attributed to the lack of an immune-suppressive component in the immune system (PMID: 25683611; 11138001). Similar to mice, genetic defects in human Foxp3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) which also known as X-linked autoimmunity-immunodeficiency syndrome (PMID: 11137993).

Publications

Species	Application	Title
mouse	FC (Intra)	Adv Sci (Weinh); Cancer-Associated Fibroblast-Induced Remodeling of Tumor Microenvironment in Recurrent Bladder Cancer; Authors - Ting Liang

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