Anticorps Monoclonal anti-Foxp3

Foxp3 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1

Réactivité testée

souris

Applications

FC

Conjugaison

FITC Fluorescent Dye

CloneNo.

3G3

N° de cat : FITC-65089

Synonymes

forkhead box P3, Foxp3, JM2, scurfin, sf

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Galerie de données de validation

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  FC experiment of mouse splenocytes using FITC-65089

  1X10^6 C57BL/6 mouse splenocytes were surface stained with APC Anti-Mouse CD4 (GK1.5) (APC-65104, Clone: GK1.5) and then treated with fixation and permeabilization reagents. Cells were then stained with 0.13 ug FITC Foxp3 antibody (FITC-65089, Clone: 3G3).

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  FC experiment of mouse splenocytes using FITC-65089

  1X10^6 C57BL/6 mouse splenocytes were surface stained with APC Anti-Mouse CD4 (GK1.5) (APC-65104, Clone: GK1.5) and then treated with fixation and permeabilization reagents. Cells were then stained with 0.13 ug FITC-mouse IgG1 isotype control antibody.

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Applications testées

• Résultats positifs en cytométrie: splénocytes de souris

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

FITC-65089 cible Foxp3 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: s/o
• Nom complet: forkhead box P3
• Numéro d’acquisition GenBank: BC132333
• Symbole du gène: Foxp3
• Identification du gène (NCBI): 20371
• Conjugaison: FITC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 494 nm / 520 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide and 0.1% gelatin
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Foxp3 (forkhead box P3), also named as IPEX, JM2 and scurfin, is a member of the forkhead transcription factor family and is essential for normal immune homeostasis (PMID: 11138001; 20429413). Foxp3 is specifically expressed in CD4+ CD25+ regulatory T cells and is crucial for their development and suppressive activity (PMID: 12612578; 12612581). In scurfy mouse, disruption of Foxp3 results in the fatal lymphoproliferative disorder attributed to the lack of an immune-suppressive component in the immune system (PMID: 25683611; 11138001). Similar to mice, genetic defects in human Foxp3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) which also known as X-linked autoimmunity-immunodeficiency syndrome (PMID: 11137993).

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