Anticorps Monoclonal anti-Foxp3

Foxp3 Monoclonal Antibody for FC (Intra)

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

souris

Applications

FC (Intra)

Conjugaison

PE Fluorescent Dye

Publications

1

CloneNo.

3G3

N° de cat : PE-65089

Synonymes

forkhead box P3, Foxp3, JM2, scurfin, sf

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Galerie de données de validation

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  FC experiment of mouse splenocytes using PE-65089

  1X10^6 unstimulated and anti-CD3/CD28-stimulated (with 50 ng/ml TGF-β, 3 days) mouse splenocytes were surface stained with CoraLite® Plus 647-conjugated Anti-Mouse CD4. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011), and then intracellularly stained with 0.06 ug PE Anti-Mouse Foxp3 (PE-65089, Clone:3G3).

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Applications testées

• Résultats positifs en cytométrie: anti-CD3/CD28-stimulated mouse splenocytes

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

PE-65089 cible Foxp3 dans les applications de FC (Intra) et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: souris
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: s/o
• Nom complet: forkhead box P3
• Numéro d’acquisition GenBank: BC132333
• Symbole du gène: Foxp3
• Identification du gène (NCBI): 20371
• Conjugaison: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.1% sodium azide and 0.5% BSA.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Foxp3 (forkhead box P3), also named as IPEX, JM2 and scurfin, is a member of the forkhead transcription factor family and is essential for normal immune homeostasis (PMID: 11138001; 20429413). Foxp3 is specifically expressed in CD4+ CD25+ regulatory T cells and is crucial for their development and suppressive activity (PMID: 12612578; 12612581). In scurfy mouse, disruption of Foxp3 results in the fatal lymphoproliferative disorder attributed to the lack of an immune-suppressive component in the immune system (PMID: 25683611; 11138001). Similar to mice, genetic defects in human Foxp3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) which also known as X-linked autoimmunity-immunodeficiency syndrome (PMID: 11137993).

Publications

Species	Application	Title
mouse	FC (Intra)	Phytomedicine; Multi-material basis and multi-mechanisms of the Dahuang Zhechong pill for regulating Treg/Th1 balance in hepatocellular carcinoma.; Authors - Li Wu

Produits associés

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011