Anticorps Polyclonal de lapin anti-Frizzled 5

• Phare
• Validé par KD/KO

Frizzled 5 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 21519-1-PBS

Synonymes

FZD5, FzE5, Fz-5, Fz 5, Frizzled-5

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Galerie de données de validation

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  WB analysis using 21519-1-AP (same clone as 21519-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21519-1-AP (Frizzled 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21519-1-PBS in a different storage buffer formulation.

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  WB analysis using 21519-1-AP (same clone as 21519-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21519-1-AP (Frizzled 5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21519-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 21519-1-AP (same clone as 21519-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21519-1-AP (Frizzled 5 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21519-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 21519-1-AP (same clone as 21519-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 21519-1-AP (FZD5 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 21519-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 21519-1-AP (same clone as 21519-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 21519-1-AP (FZD5 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 21519-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 21519-1-AP (same clone as 21519-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Frizzled 5 antibody (21519-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21519-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 21519-1-AP (same clone as 21519-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Frizzled 5 antibody (21519-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21519-1-PBS in a different storage buffer formulation.

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Informations sur le produit

21519-1-PBS cible Frizzled 5 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Frizzled 5 Protéine recombinante Ag16082
• Nom complet: frizzled homolog 5 (Drosophila)
• Masse moléculaire calculée: 585 aa, 65 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: BC114346
• Symbole du gène: FZD5
• Identification du gène (NCBI): 7855
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Fzd5, is the receptor for the Wnt5A ligand. Fzd5 modulates cell fate and cell behavior during vertebrate development. It regulates hematopoiesis by the antagonism of the canonical Wnt pathway, resulting in a pool of quiescent hematopoietic stem cells, and regulates hematopoietic stem cell function in a paracrine fashion through several distinct mechanisms including modulating canonical Wnt signaling. It acts through noncanonical Wnt signaling to promote angiogenesis. It is also involved in control cell orientation, polarity, and directional movement in response to positional cues from chemokine gradients.

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