Anticorps Monoclonal anti-Frizzled 9

Frizzled 9 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1G9G4

N° de cat : 67023-1-PBS

Synonymes

FZD9, CD349, Frizzled-9, Fz 9, Fz-9

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Galerie de données de validation

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  WB analysis of A549 using 67023-1-Ig (same clone as 67023-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 67023-1-Ig (Frizzled 9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67023-1-PBS in a different storage buffer formulation.

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  WB analysis using 67023-1-Ig (same clone as 67023-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67023-1-Ig (Frizzled 9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67023-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 67023-1-Ig (same clone as 67023-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 67023-1-Ig (Frizzled 9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67023-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 67023-1-Ig (same clone as 67023-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 67023-1-Ig (Frizzled 9 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67023-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 67023-1-Ig (same clone as 67023-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 67023-1-Ig (Frizzled 9 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67023-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 67023-1-Ig (same clone as 67023-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using Frizzled 9 antibody (67023-1-Ig, Clone: 1G9G4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67023-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67023-1-PBS cible Frizzled 9 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Frizzled 9 Protéine recombinante Ag4971
• Nom complet: frizzled homolog 9 (Drosophila)
• Masse moléculaire calculée: 591 aa, 64 kDa
• Poids moléculaire observé: 64-70 kDa
• Numéro d’acquisition GenBank: BC026333
• Symbole du gène: Frizzled 9
• Identification du gène (NCBI): 8326
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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