Anticorps Recombinant de lapin anti-G-CSF

G-CSF Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

250389H1

N° de cat : 86376-1-RR

Synonymes

CSF3, C17orf33, CSF 3, FilgrastimLenograstim, GCSF

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Galerie de données de validation

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  WB analysis using 86376-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86376-1-RR (G-CSF antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  FC experiment of K-562 using 86376-1-RR

  1x10^6 K-562 cells were intracellularly stained with 0.25 ug G-CSF Recombinant antibody (86376-1-RR, Clone:250389H1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules HepG2, cellules K-562, cellules MCF-7, cellules MOLT-4
• Résultats positifs en FC (Intra): cellules K-562,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86376-1-RR cible G-CSF dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: G-CSF Protéine recombinante HZ-1207
• Nom complet: colony stimulating factor 3 (granulocyte)
• Poids moléculaire observé: 20 kDa
• Symbole du gène: G-CSF
• Identification du gène (NCBI): 1440
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Granulocyte colony-stimulating factor (G-CSF), also referred to as CSF3, is a protective cytokine with anti-inflammatory effects. G-CSF is important in promoting survival of the granulocytic lineage cells and proliferation and migration of neutrophils as well as trophoblast cells. G-CSF acts by binding to its receptor G-CSFR (also called CSF3R), which after binding with G-CSF activates the canonical Janus kinase (Jak)/signal transducer, activator of transcription (STAT)and Ras/Raf/MAP kinase pathways. G-CSF potently stimulates the proliferation and release of peripheral blood progenitor cells into the bloodstream and is therefore used to treat neutropenia after chemotherapy. Furthermore, G-CSF levels are elevated upon intensive exercise leading to increased neutrophil counts, which are predominantly due to delayed neutrophil apoptosis.

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