Anticorps Monoclonal anti-G3BP1

• Phare
• Validé par KD/KO

G3BP1 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, RIP, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

42

CloneNo.

1E4A2

Avis (6)

N° de cat : 66486-1-Ig

Synonymes

G3BP, G3BP 1, G3BP1, HDH VIII

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Galerie de données de validation

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  WB analysis using 66486-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66486-1-Ig (G3BP1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66486-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66486-1-Ig (G3BP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 66486-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66486-1-Ig (G3BP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 66486-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66486-1-Ig (G3BP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 66486-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66486-1-Ig (G3BP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 66486-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66486-1-Ig (G3BP antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 66486-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66486-1-Ig (G3BP antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human lymphoma using 66486-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66486-1-Ig (G3BP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 66486-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66486-1-Ig (G3BP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 66486-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66486-1-Ig (G3BP antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 66486-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66486-1-Ig (G3BP antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of rat brain using 66486-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66486-1-Ig (G3BP1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 66486-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66486-1-Ig (G3BP1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 66486-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using G3BP1 antibody (66486-1-Ig, Clone: 1E4A2 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules 4T1, cellules HEK-293, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules NIH/3T3, cellules PC-12, cellules RAW 264.7, tissu cérébral de porc, tissu cérébral de souris
• Résultats positifs en IHC: tissu testiculaire humain, tissu cérébral de rat, tissu de côlon humain, tissu de lymphome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: sodium arsenite treated HeLa cells,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 21 publications below
• IF: See 31 publications below
• IP: See 2 publications below
• FC: See 2 publications below
• CoIP: See 4 publications below
• RIP: See 1 publications below

Informations sur le produit

66486-1-Ig cible G3BP1 dans les applications de WB, RIP, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: G3BP1 Protéine recombinante Ag3728
• Nom complet: GTPase activating protein (SH3 domain) binding protein 1
• Masse moléculaire calculée: 466 aa, 52 kDa
• Poids moléculaire observé: 68 kDa
• Numéro d’acquisition GenBank: BC006997
• Symbole du gène: G3BP1
• Identification du gène (NCBI): 10146
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GAP SH3 Binding Protein 1 (G3BP1), also named as G3BP, is an effector of stress granule (SG) assembly. SG biology plays an important role in the pathophysiology of TDP-43 in ALS and FTLD-U. G3BP1 can be used as a marker of SG. It has been shown to function downstream of Ras and play a role in RNA metabolism, signal transduction, and proliferation. G3BP1 is a ubiquitously expressed protein that localizes to the cytoplasm in proliferating cells and to the nucleus in non-proliferating cells. G3BP1 has recently been implicated in cancer biology.

Publications

Species	Application	Title
mouse	WB,IF	Nature; DDX3X acts as a live-or-die checkpoint in stressed cells by regulating NLRP3 inflammasome.; Authors - Parimal Samir
mouse	IF	Nature; Galactosaminogalactan activates the inflammasome to provide host protection.; Authors - Benoit Briard
human	IF	Sci Transl Med; Precise genomic editing of pathogenic mutations in RBM20 rescues dilated cardiomyopathy; Authors - Takahiko Nishiyama
mouse	IF	Nat Struct Mol Biol; TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.; Authors - Xinxin Zuo
human	WB,IF,IP	Autophagy; Stress granule homeostasis is modulated by TRIM21-mediated ubiquitination of G3BP1 and autophagy-dependent elimination of stress granules; Authors - Cuiwei Yang
human	IF	Nat Chem Biol; K29-linked ubiquitin signaling regulates proteotoxic stress response and cell cycle.; Authors - Yuanyuan Yu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Haibo (Verified Customer) (10-26-2020)

This is a excellent antibody to visualize stress granules tested in HEK293 and human fibroblasts.

• Applications: Immunofluorescence
• Cell Tissue Type: HEK293 and human fibroblasts

FH

Manohar (Verified Customer) (09-23-2020)

1% milk is used

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293

FH

David (Verified Customer) (01-13-2020)

Good signal in control cells and immunopositive puncta seen in response to stress (e.g. sodium arsenite).

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: SH-SY5Y

FH

Azita (Verified Customer) (10-04-2019)

Assembly of stress granula upon treatment with sodium arsenite for 40 min. (It works great)

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1/400
• Cell Tissue Type: NSC-34

G3BP1 Antibody Immunofluorescence, validation (1/400 dilution) in NSC-34 (Cat no:66486-1-Ig)

FH

Kyosuke (Verified Customer) (06-12-2019)

It works well on HEK293T for Western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HEK293T

FH

Natalia (Verified Customer) (06-06-2019)

PFA fixated cells

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Hepa1-6 cell line

G3BP1 Antibody Immunofluorescence, validation (1:1000 dilution) in Hepa1-6 cell line (Cat no:66486-1-Ig)