Anticorps Recombinant de lapin anti-G3BP1

G3BP1 Recombinant Antibody for IHC, IF/ICC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

IHC, IF/ICC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

7F14

N° de cat : 80777-1-PBS

Synonymes

7F14, ATP-dependent DNA helicase VIII, EC:3.6.4.12, G3BP, G3BP 1

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Galerie de données de validation

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  IHC staining of mouse brain using 80777-1-RR (same clone as 80777-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80777-1-RR (G3BP1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80777-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 80777-1-RR (same clone as 80777-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80777-1-RR (G3BP1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80777-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 80777-1-RR (same clone as 80777-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using G3BP1 antibody (80777-1-RR, Clone: 7F14 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80777-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 80777-1-RR (same clone as 80777-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using G3BP1 antibody (80777-1-RR, Clone: 7F14 ) at dilution of 1:1400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80777-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 80777-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80777-1-RR against Human G3BP1 were performed. The affinity constant is 7.07 nM.

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Informations sur le produit

80777-1-PBS cible G3BP1 dans les applications de IHC, IF/ICC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: G3BP1 Protéine recombinante Ag3728
• Nom complet: GTPase activating protein (SH3 domain) binding protein 1
• Masse moléculaire calculée: 466 aa, 52 kDa
• Numéro d’acquisition GenBank: BC006997
• Symbole du gène: G3BP1
• Identification du gène (NCBI): 10146
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GAP SH3 Binding Protein 1 (G3BP1), also named as G3BP, is an effector of stress granule (SG) assembly. SG biology plays an important role in the pathophysiology of TDP-43 in ALS and FTLD-U. G3BP1 can be used as a marker of SG. It has been shown to function downstream of Ras and play a role in RNA metabolism, signal transduction, and proliferation. G3BP1 is a ubiquitously expressed protein that localizes to the cytoplasm in proliferating cells and to the nucleus in non-proliferating cells. G3BP1 has recently been implicated in cancer biology.

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