Anticorps Monoclonal anti-G3BP2

• Phare
• Validé par KD/KO

G3BP2 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2E5G3

N° de cat : 68580-1-PBS

Synonymes

G3BP 2, 2E5G3, Ras GTPase-activating protein-binding protein 2, GAP SH3 domain-binding protein 2, GAP SH3 domain binding protein 2

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Galerie de données de validation

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  WB analysis of SCaBER using 68580-1-Ig (same clone as 68580-1-PBS)

  SCaBER cells were subjected to SDS PAGE followed by western blot with 68580-1-Ig (G3BP2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68580-1-PBS in a different storage buffer formulation.

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  WB analysis using 68580-1-Ig (same clone as 68580-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68580-1-Ig (G3BP2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68580-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 68580-1-Ig (same clone as 68580-1-PBS)

  WB result of G3BP2 antibody (68580-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-G3BP2 transfected HepG2 cells. This data was developed using the same antibody clone with 68580-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 68580-1-Ig (same clone as 68580-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 68580-1-Ig (G3BP2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68580-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 68580-1-Ig (same clone as 68580-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 68580-1-Ig (G3BP2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68580-1-PBS in a different storage buffer formulation.

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  WB analysis of HCT 116 using 68580-1-Ig (same clone as 68580-1-PBS)

  HCT 116 cells were subjected to SDS PAGE followed by western blot with 68580-1-Ig (G3BP2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68580-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68580-1-Ig (same clone as 68580-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68580-1-Ig (G3BP2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68580-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68580-1-Ig (same clone as 68580-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using G3BP2 antibody (68580-1-Ig, Clone: 2E5G3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 68580-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68580-1-PBS cible G3BP2 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: G3BP2 Protéine recombinante Ag9222
• Nom complet: GTPase activating protein (SH3 domain) binding protein 2
• Masse moléculaire calculée: 482aa,54 kDa; 449aa,51 kDa
• Poids moléculaire observé: 54 kDa
• Numéro d’acquisition GenBank: BC011731
• Symbole du gène: G3BP2
• Identification du gène (NCBI): 9908
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Stress granules (SGs) are cytoplasmic mRNA-protein condensates formed in response to cellular stressors, such as oxidative stress, ultraviolet radiation, and viral infection (1). The Ras-GTPase-activating protein-binding proteins (G3BPs), consisting of G3BP1 and G3BP2, are key nucleating factors essential for SG formation. They function to protect RNAs from harmful conditions. G3BP2 is mainly distributed in the cytoplasm and participates in the formation of stress granules, cell differentiation, proliferation, and signal transduction. Accumulating evidence has demonstrated that aberrant expression of G3BP2 contributes to cancer initiation and progression, such as high expression of G3BP2 increasing cell stemness, metastasis and chemoresistance in breast cancer.

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