Anticorps Recombinant de lapin anti-G3BP2

• Phare
• Validé par KD/KO

G3BP2 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

230275G3

N° de cat : 82080-6-RR

Synonymes

G3BP 2, 230275G3, G3BP-2, GAP SH3 domain binding protein 2, GAP SH3 domain-binding protein 2

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Galerie de données de validation

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  WB analysis of HepG2 using 82080-6-RR

  WB result of G3BP2 antibody (82080-6-RR; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-G3BP2 transfected HepG2 cells.

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  WB analysis using 82080-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82080-6-RR (G3BP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human intrahepatic cholangiocarcinoma using 82080-6-RR

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82080-6-RR (G3BP2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 82080-6-RR

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82080-6-RR (G3BP2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 82080-6-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 82080-6-RR (G3BP2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 82080-6-RR

  Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using G3BP2 antibody (82080-6-RR, Clone: 230275G3 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  FC experiment of U2OS using 82080-6-RR

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug Anti-Human G3BP2 (82080-6-RR, Clone:230275G3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 82080-6-RR

  Biolayer interferometry (BLl) kinetic assays of 82080-6-RR against Human G3BP2 were performed. The affinity constant is 4.69 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules HEK-293, cellules HepG2, cellules Jurkat, cellules K-562, cellules MCF-7, tissu de cervelet de souris
• Résultats positifs en IHC: human intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: sodium arsenite treated HeLa cells,
• Résultats positifs en FC (Intra): cellules U2OS

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82080-6-RR cible G3BP2 dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: G3BP2 Protéine recombinante Ag9355
• Nom complet: GTPase activating protein (SH3 domain) binding protein 2
• Masse moléculaire calculée: 482aa,54 kDa; 449aa,51 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: BC011731
• Symbole du gène: G3BP2
• Identification du gène (NCBI): 9908
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Stress granules (SGs) are cytoplasmic mRNA-protein condensates formed in response to cellular stressors, such as oxidative stress, ultraviolet radiation, and viral infection (1). The Ras-GTPase-activating protein-binding proteins (G3BPs), consisting of G3BP1 and G3BP2, are key nucleating factors essential for SG formation. They function to protect RNAs from harmful conditions. G3BP2 is mainly distributed in the cytoplasm and participates in the formation of stress granules, cell differentiation, proliferation, and signal transduction. Accumulating evidence has demonstrated that aberrant expression of G3BP2 contributes to cancer initiation and progression, such as high expression of G3BP2 increasing cell stemness, metastasis and chemoresistance in breast cancer.

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