Anticorps Polyclonal de lapin anti-G6PC

G6PC Polyclonal Antibody for WB, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF-P, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 29084-1-PBS

Synonymes

G6PC1, EC:3.1.3.9, G6Pase, G-6-Pase, G6Pase-alpha

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Galerie de données de validation

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  WB analysis using 29084-1-AP (same clone as 29084-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29084-1-AP (G6PC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29084-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse liver using 29084-1-AP (same clone as 29084-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using G6PC antibody (29084-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29084-1-PBS in a different storage buffer formulation.

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Informations sur le produit

29084-1-PBS cible G6PC dans les applications de WB, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: G6PC Protéine recombinante Ag30614
• Nom complet: glucose-6-phosphatase, catalytic subunit
• Masse moléculaire calculée: 357 aa, 40 kDa
• Poids moléculaire observé: 40 kDa
• Numéro d’acquisition GenBank: BC130478
• Symbole du gène: G6PC
• Identification du gène (NCBI): 2538
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Glucose-6-phosphatase-α (G6PC) is a key enzyme in glucose homeostasis that catalyzes the hydrolysis of glucose-6-phosphate to glucose and phosphate in the terminal step of gluconeogenesis and glycogenolysis. G6PC activity is restricted to the liver , the kidney cortex and the small intestine and confers on these three organs the capacity to release glucose into the systemic circulation (PMID: 21983240).The encoded enzyme is anchored to the ER by nine transmembrane helices with the amino (N)-terminus in the lumen and the carboxyl (C)-terminus in the cytoplasm (PMID: 15542400).

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