Anticorps Monoclonal anti-G6PD

• Phare
• Validé par KD/KO

G6PD Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

2A7B12

N° de cat : 66373-1-PBS

Synonymes

2A7B12, EC:1.1.1.49, G6P, G6PD1, Glucose-6-phosphate 1-dehydrogenase

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Galerie de données de validation

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  WB analysis of HeLa using 66373-1-Ig (same clone as 66373-1-PBS)

  WB result of G6PD antibody (66373-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-G6PD transfected HeLa cells. This data was developed using the same antibody clone with 66373-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66373-1-Ig (same clone as 66373-1-PBS)

  Several cell lines were subjected to SDS PAGE followed by western blot with 66373-1-Ig (G6PD Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66373-1-PBS in a different storage buffer formulation.

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  WB analysis of rat spleen using 66373-1-Ig (same clone as 66373-1-PBS)

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 66373-1-Ig (G6PD Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66373-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66373-1-Ig (same clone as 66373-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66373-1-Ig (G6PD Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66373-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66373-1-Ig (same clone as 66373-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66373-1-Ig (G6PD Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66373-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66373-1-Ig (same clone as 66373-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using G6PD antibody (66373-1-Ig, Clone: 2A7B12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66373-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66373-1-Ig (same clone as 66373-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66373-1-Ig (G6PD antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66373-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66373-1-PBS cible G6PD dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: G6PD Protéine recombinante Ag21862
• Nom complet: glucose-6-phosphate dehydrogenase
• Masse moléculaire calculée: 59 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC000337
• Symbole du gène: G6PD
• Identification du gène (NCBI): 2539
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

G6PD(Glucose-6-phosphate 1-dehydrogenase) belongs to the glucose-6-phosphate dehydrogenase family. It is a housekeeping enzyme encoded in mammals by an X-linked gene. G6PD has important functions in intermediary metabolism because it catalyzes the first step in the pentose phosphate pathway and provides reductive potential in the form of NADPH.(PMID:7489710). This protein has 3 isoforms produced by alternative splicing.

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