Anticorps Polyclonal de lapin anti-GAA

GAA Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 29993-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 29993-1-AP (same clone as 29993-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29993-1-AP (GAA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29993-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 29993-1-AP (same clone as 29993-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 29993-1-AP (GAA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29993-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 29993-1-AP (same clone as 29993-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 29993-1-AP (GAA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29993-1-PBS in a different storage buffer formulation.

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Informations sur le produit

29993-1-PBS cible GAA dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GAA Protéine recombinante Ag28970
• Nom complet: glucosidase, alpha; acid
• Masse moléculaire calculée: 105 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: BC040431
• Symbole du gène: GAA
• Identification du gène (NCBI): 2548
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GAA (Alpha-1,4-glucosidase) is a lysosomal enzyme involved in the degradation of glycogen within cellular vacuoles. After translation, GAA undergoes proteolytic processing to form two lengths of lysosomal α-glucosidase, and both N-terminal and C-terminal processing occur. Typically, GAA is synthesized as an immature glycoprotein precursor (110 kDa) in the endoplasmic reticulum and undergoes a seriesof proteolytic and N-glycan processing events to yield an intermediate (95 kDa) and two lysosomal (76 and 70 kDa) isoforms. This antibody can detect the 110 kDa precursor form.

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