Anticorps Polyclonal de lapin anti-GAB2

• Phare
• Validé par KD/KO

GAB2 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 29272-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 29272-1-AP (same clone as 29272-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 29272-1-AP (GAB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29272-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 29272-1-AP (same clone as 29272-1-PBS)

  WB result of GAB2 antibody (29272-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GAB2 transfected K-562 cells. This data was developed using the same antibody clone with 29272-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 29272-1-AP (same clone as 29272-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29272-1-AP (GAB2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29272-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 29272-1-AP (same clone as 29272-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29272-1-AP (GAB2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29272-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 29272-1-AP (same clone as 29272-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 29272-1-AP (GAB2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29272-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 29272-1-AP (same clone as 29272-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 29272-1-AP (GAB2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29272-1-PBS in a different storage buffer formulation.

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Informations sur le produit

29272-1-PBS cible GAB2 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GAB2 Protéine recombinante Ag30347
• Nom complet: GRB2-associated binding protein 2
• Masse moléculaire calculée: 676 aa, 74 kDa
• Poids moléculaire observé: 74-90 kDa
• Numéro d’acquisition GenBank: BC131711
• Symbole du gène: GAB2
• Identification du gène (NCBI): 9846
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Growth factor receptor-bound protein 2 (GAB2), an adaptor protein, belongs to the Gab family and is involved in various biologic processes. Gab2 mainly mediates phosphatidylinositol 3-kinase (PI3K) and extracellular signal-regulated kinase (ERK) signaling pathways. Furthermore, Gab2 governs tumorigenic signaling and is a novel potential therapeutic target in leukemia, breast cancer, ovarian cancer, and melanoma. Gab2 is the major 98-kDa phosphoprotein associated with SHP-2, PI 3-kinase, and Grb2 in normal human lymphocytes. (PMID: 32269703, PMID: 28842424, PMID: 28420432, PMID: 10849428)

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