Anticorps Monoclonal anti-GABARAPL1

• Phare
• Validé par KD/KO

GABARAPL1 Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

1E10F6

N° de cat : 66458-1-PBS

Synonymes

1E10F6, APG8 LIKE, APG8L, ATG8, ATG8L

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Galerie de données de validation

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  WB analysis of mouse brain using 66458-1-Ig (same clone as 66458-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66458-1-Ig (GABARAPL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66458-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 66458-1-Ig (same clone as 66458-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66458-1-Ig (GABARAPL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66458-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig brain using 66458-1-Ig (same clone as 66458-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66458-1-Ig (GABARAPL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66458-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66458-1-Ig (same clone as 66458-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GABARAPL1 antibody (66458-1-Ig, Clone: 1E10F6 ) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66458-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66458-1-PBS cible GABARAPL1 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GABARAPL1 Protéine recombinante Ag1473
• Nom complet: GABA(A) receptor-associated protein like 1
• Masse moléculaire calculée: 14 kDa
• Poids moléculaire observé: 16-18 kDa
• Numéro d’acquisition GenBank: BC009309
• Symbole du gène: GABARAPL1
• Identification du gène (NCBI): 23710
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GABARAPL1 (GABARAP-like protein 1), also named ATG8, GEC1, APG8L, ATG8L, and APG8-LIKE, is a member of the GABARP (GABAA receptor-associated protein) family. GABARAPL1 was initially identified as an estrogen-regulated gene and the protein acts in receptor and vesicle transport. It's also involved in the process of autophagy like GABARAP and GABARAPL2 and may be considered as an autophagic marker. It is expressed at very high levels in the brain, heart, peripheral blood leukocytes, liver, kidney, placenta, and skeletal muscle and at very low levels in the thymus and small intestine.

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