Anticorps Monoclonal anti-GABPA

GABPA Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2A7E3

N° de cat : 68645-1-PBS

Synonymes

GABP subunit alpha, GA-binding protein alpha chain, GA binding protein alpha chain, E4TF1A, E4TF1 60

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Galerie de données de validation

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  WB analysis using 68645-1-Ig (same clone as 68645-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68645-1-Ig (GABPA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68645-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 68645-1-Ig (same clone as 68645-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using GABPA antibody (68645-1-Ig, Clone: 2A7E3 ) at dilution of 1:2000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red). This data was developed using the same antibody clone with 68645-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68645-1-PBS cible GABPA dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GABPA Protéine recombinante Ag33705
• Nom complet: GA binding protein transcription factor, alpha subunit 60kDa
• Masse moléculaire calculée: 454 aa, 51 kDa
• Poids moléculaire observé: 56-60 kDa
• Numéro d’acquisition GenBank: BC035031
• Symbole du gène: GABPA
• Identification du gène (NCBI): 2551
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GA-binding protein alpha chain (GABP alpha subunit, GABPA, nuclear respiratory factor 2 subunit alpha, transcription factor E4TF1-60) is one of three GA-binding protein transcription factor subunits which functions as a DNA-binding subunit.GABPA is a member of Ets family, binds to the Yap promoter and activates YAP transcription(23684612). Since this subunit shares identity with a subunit encoding the nuclear respiratory factor 2 gene, it is likely involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. This subunit also shares identity with a subunit constituting the transcription factor E4TF1, responsible for expression of the adenovirus E4 gene. Because of its chromosomal localization and ability to form heterodimers with other polypeptides, this gene may play a role in the Down Syndrome phenotype.

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