Anticorps Recombinant de lapin anti-GABPA

GABPA Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

242578F10

N° de cat : 84951-4-RR

Synonymes

242578F10, E4TF1 60, E4TF1A, GA binding protein alpha chain, GA-binding protein alpha chain

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Galerie de données de validation

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  WB analysis using 84951-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84951-4-RR (GABPA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 84951-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84951-4-RR (GABPA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 84951-4-RR

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 84951-4-RR (GABPA antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 84951-4-RR

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 84951-4-RR (GABPA antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of NIH/3T3 using 84951-4-RR

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using GABPA antibody (84951-4-RR, Clone: 242578F10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of HeLa using 84951-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GABPA antibody (84951-4-RR, Clone: 242578F10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of A431 using 84951-4-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using GABPA antibody (84951-4-RR, Clone: 242578F10 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of HeLa using 84951-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GABPA antibody (84951-4-RR, Clone: 242578F10 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 84951-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84951-4-RR against Human GABPA were performed. The affinity constant is 2.51 nM.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HuH-7, cellules K-562, cellules NIH/3T3, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules NIH/3T3, cellules A431, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84951-4-RR cible GABPA dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: GABPA Protéine recombinante Ag16191
• Nom complet: GA binding protein transcription factor, alpha subunit 60kDa
• Masse moléculaire calculée: 454 aa, 51 kDa
• Poids moléculaire observé: 56-60 kDa
• Numéro d’acquisition GenBank: BC035031
• Symbole du gène: GABPA
• Identification du gène (NCBI): 2551
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GA-binding protein alpha chain (GABP alpha subunit, GABPA, nuclear respiratory factor 2 subunit alpha, transcription factor E4TF1-60) is one of three GA-binding protein transcription factor subunits which functions as a DNA-binding subunit.GABPA is a member of Ets family, binds to the Yap promoter and activates YAP transcription(23684612). Since this subunit shares identity with a subunit encoding the nuclear respiratory factor 2 gene, it is likely involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. This subunit also shares identity with a subunit constituting the transcription factor E4TF1, responsible for expression of the adenovirus E4 gene. Because of its chromosomal localization and ability to form heterodimers with other polypeptides, this gene may play a role in the Down Syndrome phenotype.

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