Anticorps Polyclonal de lapin anti-GABRG2

GABRG2 Polyclonal Antibody for FC, IF, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 14104-1-AP

Synonymes

CAE2, ECA2, GABA A receptor gamma 2, GABAA receptor gamma 2, GABRG2, GEFSP3

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Galerie de données de validation

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  WB analysis using 14104-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14104-1-AP (GABRG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 14104-1-AP

  IP Result of anti-GABRG2 (IP:14104-1-AP, 4ug; Detection:14104-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IF Staining of mouse cerebellum using 14104-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using GABRG2 antibody (14104-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of SH-SY5Y using 14104-1-AP

  1X10^6 SH-SY5Y cells were stained with 0.2ug GABRG2 antibody (14104-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: cellules Neuro-2a, tissu cérébral de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IF: tissu de cervelet de souris,
• Résultats positifs en cytométrie: cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Informations sur le produit

14104-1-AP cible GABRG2 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GABRG2 Protéine recombinante Ag5237
• Nom complet: gamma-aminobutyric acid (GABA) A receptor, gamma 2
• Masse moléculaire calculée: 467 aa, 54 kDa
• Poids moléculaire observé: 54-60 kDa
• Numéro d’acquisition GenBank: BC059389
• Symbole du gène: GABRG2
• Identification du gène (NCBI): 2566
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GABRG2 (gamma-aminobutyric acid type A receptor subunit gamma2), which is expected to be located in cell membrane and expressed in brain and retina. The calculated molecular weight of the protein is 54 kDa. This antibody can recognize the 43 kDa band of target (PMID:12091434). And it also has glycosylation modification. GABA is the major inhibitory neurotransmitter in the mammlian brain, where it acts at GABA-A receptors, which are ligand-gated chloride channels. GABA-A receptors are pentameric, consisting of proteins from several subunit classes: alpha, beta, gamma, delta and rho. Mutations in this gene have been associated with epilepsy and febrile seizures.

Publications

Species	Application	Title
mouse	WB	Cell Death Dis; Transgenic overexpression of furin increases epileptic susceptibility.; Authors - Yi Yang
mouse	WB,IP	CNS Neurosci Ther; STK24 modulates excitatory synaptic transmission in epileptic hippocampal neurons.; Authors - Juan Yang
rat	IHC, IF	Neurosci Lett; Acupuncture Improves Locomotor Function by Enhancing GABA receptor Expression in Transient Focal Cerebral Ischemia Rats.; Authors - Qian Xu
mouse	WB,IF	Neuropharmacology; A role of GABAA receptor α1 subunit in the hippocampus for rapid-acting antidepressant-like effects of ketamine; Authors - Xiao-Hui Tang
mouse	WB	Front Endocrinol (Lausanne); Altered GnRH neuron and ovarian innervation characterize reproductive dysfunction linked to the Fragile X messenger ribonucleoprotein (Fmr1) gene mutation; Authors - Pedro A Villa
human	WB,IF	Nat Genet; Pan-cancer analysis identifies tumor-specific antigens derived from transposable elements; Authors - Nakul M Shah

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