Anticorps Polyclonal de lapin anti-GABRG2

GABRG2 Polyclonal Antibody for WB, IF-P, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF-P, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14104-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 14104-1-AP (same clone as 14104-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14104-1-AP (GABRG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14104-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 14104-1-AP (same clone as 14104-1-PBS)

  IP result of anti-GABRG2 (IP:14104-1-AP, 4ug; Detection:14104-1-AP 1:500) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 14104-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse cerebellum using 14104-1-AP (same clone as 14104-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using GABRG2 antibody (14104-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14104-1-PBS in a different storage buffer formulation.

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Informations sur le produit

14104-1-PBS cible GABRG2 dans les applications de WB, IF-P, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GABRG2 Protéine recombinante Ag5237
• Nom complet: gamma-aminobutyric acid (GABA) A receptor, gamma 2
• Masse moléculaire calculée: 467 aa, 54 kDa
• Poids moléculaire observé: 54-60 kDa
• Numéro d’acquisition GenBank: BC059389
• Symbole du gène: GABRG2
• Identification du gène (NCBI): 2566
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GABRG2 (gamma-aminobutyric acid type A receptor subunit gamma2), which is expected to be located in cell membrane and expressed in brain and retina. The calculated molecular weight of the protein is 54 kDa. This antibody can recognize the 43 kDa band of target (PMID:12091434). And it also has glycosylation modification. GABA is the major inhibitory neurotransmitter in the mammlian brain, where it acts at GABA-A receptors, which are ligand-gated chloride channels. GABA-A receptors are pentameric, consisting of proteins from several subunit classes: alpha, beta, gamma, delta and rho. Mutations in this gene have been associated with epilepsy and febrile seizures.

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