Anticorps Polyclonal de lapin anti-GAD1

• Phare
• Validé par KD/KO

GAD1 Polyclonal Antibody for WB, IHC, IF-Fro, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-Fro, IP, ELISA

Conjugaison

Non conjugué

N° de cat : 10408-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 10408-1-AP (same clone as 10408-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10408-1-AP (GAD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10408-1-PBS in a different storage buffer formulation.

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  WB analysis using 10408-1-AP (same clone as 10408-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10408-1-AP (GAD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10408-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 10408-1-AP (same clone as 10408-1-PBS)

  IP result of anti-GAD1 (IP:10408-1-AP, 4ug; Detection:10408-1-AP 1:500) with mouse brain tissue lysate 1920 ug. This data was developed using the same antibody clone with 10408-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 10408-1-AP (same clone as 10408-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10408-1-AP (GAD1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10408-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 10408-1-AP (same clone as 10408-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10408-1-AP (GAD1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10408-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 10408-1-AP (same clone as 10408-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10408-1-AP (GAD1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10408-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 10408-1-AP (same clone as 10408-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10408-1-AP (GAD1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10408-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 10408-1-AP (same clone as 10408-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 10408-1-AP (GAD1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10408-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 10408-1-AP (same clone as 10408-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 10408-1-AP (GAD1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10408-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 10408-1-AP (same clone as 10408-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using GAD1 antibody (10408-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 10408-1-PBS in a different storage buffer formulation.

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Informations sur le produit

10408-1-PBS cible GAD1 dans les applications de WB, IHC, IF-Fro, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GAD1 Protéine recombinante Ag0666
• Nom complet: glutamate decarboxylase 1 (brain, 67kDa)
• Masse moléculaire calculée: 67 kDa
• Poids moléculaire observé: 67 kDa
• Numéro d’acquisition GenBank: BC002815
• Symbole du gène: GAD1
• Identification du gène (NCBI): 2571
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The GAD1(glutamate decarboxylase 1) gene encodes 67 kDa glutamic acid decarboxylase isoform (GAD67), the rate-limiting enzyme responsible for γ-aminobutyric acid (GABA) biosynthesis from glutamatic acid and the major GAD isoform in the human brain for early brain development (PMID:21302352). It is also potentially involved in variety of skin activities. It belongs to the group II decarboxylase family.

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