Anticorps Monoclonal anti-GAD1

GAD1 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(7)

CloneNo.

2D5B7

Avis (1)

N° de cat : 67648-1-Ig

Synonymes

2D5B7, EC:4.1.1.15, FLJ45882, GAD, GAD 1

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Galerie de données de validation

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  WB analysis using 67648-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67648-1-Ig (GAD1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis of pig brain using 67648-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67648-1-Ig (GAD1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67648-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67648-1-Ig (GAD1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 67648-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67648-1-Ig (GAD1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat cerebellum using 67648-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67648-1-Ig (GAD1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 67648-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67648-1-Ig (GAD1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 67648-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67648-1-Ig (GAD1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat cerebellum using 67648-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67648-1-Ig (GAD1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat brain using 67648-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using GAD1 antibody (67648-1-Ig, Clone: 2D5B7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de porc, tissu cérébral de lapin, tissu cérébral de rat, tissu cérébral de souris, tissu de cervelet de lapin, tissu de cervelet de porc, tissu de cervelet de rat, tissu de cervelet de souris
• Résultats positifs en IHC: tissu de cervelet de rat, tissu de cervelet de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de rat,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 4 publications below

Informations sur le produit

67648-1-Ig cible GAD1 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GAD1 Protéine recombinante Ag21336
• Nom complet: glutamate decarboxylase 1 (brain, 67kDa)
• Masse moléculaire calculée: 67 kDa
• Poids moléculaire observé: 67 kDa, 57 kDa
• Numéro d’acquisition GenBank: BC002815
• Symbole du gène: GAD1
• Identification du gène (NCBI): 2571
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GAD1, also named as GAD67, belongs to the group II decarboxylase family. GAD1 is the rate-limiting enzyme responsible for γ-aminobutyric acid (GABA) biosynthesis from glutamatic acid and the major GAD isoform in the human brain for early brain development (PMID:21302352). It is also potentially involved in variety of skin activities.

Publications

Species	Application	Title
mouse	WB	Biol Trace Elem Res; Effect of Voluntary Wheel Running on Anxiety- and Depression-Like Behaviors in Fluoride-Exposed Mice; Authors - Mengjie Qi
rat	IHC	Acta Histochem; Proposal for a simple and easy-to-implement protocol for three-dimensional tissue imaging that is compatible with observation using a confocal microscope; Authors - Takuto Matano
human	WB	Dev Cell; The de novo synthesis of GABA and its gene regulatory function control hepatocellular carcinoma metastasis; Authors - Li Li
mouse	IF	Acta Pharmacol Sin; Differential regulation of pruritic sensation and emotion by cannabinoid type 1 receptors on mPFC glutamatergic and GABAergic neurons; Authors - Ou-Yang Zhanmu
mouse	IF	J Psychiatr Res; NLRP3 in the GABAergic neuron induces cognitive impairments in a mouse model of hemorrhage shock and resuscitation; Authors - Ji-Zhen Liu
mouse,human	WB,IF	Cells; CREB1 Facilitates GABAergic Neural Differentiation of Human Mesenchymal Stem Cells through BRN2 for Pain Alleviation and Locomotion Recovery after Spinal Cord Injury; Authors - Yanbing Kao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Reyes (Verified Customer) (07-29-2024)

This is the first time a GAD antibody (in green) works properly in a human brain FFPE tissue.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Brain Cortex

GAD1 Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:67648-1-Ig)

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