Anticorps Monoclonal anti-GAD1

GAD1 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2D5B7

N° de cat : 67648-1-PBS

Synonymes

2D5B7, EC:4.1.1.15, FLJ45882, GAD, GAD 1

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Galerie de données de validation

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  WB analysis of pig brain using 67648-1-Ig (same clone as 67648-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67648-1-Ig (GAD1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67648-1-PBS in a different storage buffer formulation.

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  WB analysis using 67648-1-Ig (same clone as 67648-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67648-1-Ig (GAD1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67648-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67648-1-Ig (same clone as 67648-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67648-1-Ig (GAD1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67648-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 67648-1-Ig (same clone as 67648-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67648-1-Ig (GAD1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67648-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat cerebellum using 67648-1-Ig (same clone as 67648-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67648-1-Ig (GAD1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67648-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 67648-1-Ig (same clone as 67648-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67648-1-Ig (GAD1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67648-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 67648-1-Ig (same clone as 67648-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67648-1-Ig (GAD1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67648-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat cerebellum using 67648-1-Ig (same clone as 67648-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67648-1-Ig (GAD1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67648-1-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 67648-1-Ig (same clone as 67648-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using GAD1 antibody (67648-1-Ig, Clone: 2D5B7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67648-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67648-1-PBS cible GAD1 dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GAD1 Protéine recombinante Ag21336
• Nom complet: glutamate decarboxylase 1 (brain, 67kDa)
• Masse moléculaire calculée: 67 kDa
• Poids moléculaire observé: 67 kDa, 57 kDa
• Numéro d’acquisition GenBank: BC002815
• Symbole du gène: GAD1
• Identification du gène (NCBI): 2571
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GAD1, also named as GAD67, belongs to the group II decarboxylase family. GAD1 is the rate-limiting enzyme responsible for γ-aminobutyric acid (GABA) biosynthesis from glutamatic acid and the major GAD isoform in the human brain for early brain development (PMID:21302352). It is also potentially involved in variety of skin activities.

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