Anticorps Polyclonal de lapin anti-GAD65

GAD65 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(23)

Avis (2)

N° de cat : 20746-1-AP

Synonymes

GAD2, GAD-65, GAD 65, EC:4.1.1.15

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Galerie de données de validation

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  WB analysis using 20746-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20746-1-AP (GAD65 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 20746-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20746-1-AP (GAD65 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 20746-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20746-1-AP (GAD65 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 20746-1-AP

  IP result of anti-GAD65 (IP:20746-1-AP, 4ug; Detection:20746-1-AP 1:1000) with mouse brain tissue lysate 3440ug.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat
• Résultats positifs en IP: tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:3000-1:20000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 16 publications below
• IHC: See 3 publications below
• IF: See 4 publications below

Informations sur le produit

20746-1-AP cible GAD65 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)
• Masse moléculaire calculée: 65 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: NM_000818
• Symbole du gène: GAD65
• Identification du gène (NCBI): 2572
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GAD2, also named as GAD65, belongs to the group II decarboxylase family. GAD2 catalyzes the production of GABA. It is responsible for the synthesis of the essential neurotransmitter gamma-aminobutyric acid (GABA) from L-glutamic acid. GAD2 is expressed in nervous and endocrine systems and are thought to be involved in synaptic transmission and INS secretion. Autoantibodies against GAD2 may serve as markers for type I diabetes. Many individuals suffering from an adult onset disorder known as Stiff Person Syndrome (SPS) also express autoantibodies to GAD2. The antibody is specific to GAD2.

Publications

Species	Application	Title
human,mouse	WB	Nat Cell Biol; Cancer-cell-derived GABA promotes β-catenin-mediated tumour growth and immunosuppression.; Authors - De Huang
mouse	WB	Neurobiol Stress; A sex-dependent delayed maturation of visual plasticity induced by adverse experiences in early childhood.; Authors - Yueqin Liu
mouse	WB	Aging (Albany NY); A five-m6A regulatory gene signature is a prognostic biomarker in lung adenocarcinoma patients.; Authors - Xiao Wu
mouse	WB	Aging (Albany NY); Maternal high sugar and fat diet benefits offspring brain function via targeting on the gut-brain axis.; Authors - Dongdong Wang
mouse	WB	CNS Neurosci Ther; Integrin α3 is required for high-frequency repetitive transcranial magnetic stimulation-induced glutamatergic synaptic transmission in mice with ischemia; Authors - Li Liu
		Transl Psychiatry; A diet high in sugar and fat influences neurotransmitter metabolism and then affects brain function by altering the gut microbiota.; Authors - Yinrui Guo

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Reyes (Verified Customer) (08-06-2024)

GAD2/GAD65 (green) is supposed to mark cytoplasmatically or puncta-like my inhibitory neurons, but this lot did not seem to work, as it seems only artifacts marking around the nuclei.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: Human Brain Cortex

GAD65 Antibody Immunofluorescence validation (1:50 dilution) in Human Brain Cortex (Cat no:20746-1-AP)

FH

Lana (Verified Customer) (02-06-2020)

SDS-PAGE: 15 ug/ul RIPA lysate of whole striatal tissue. 4-12% Bis-tris gradient gel.Transfer: Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4CBlocking: SEA Block Blocking Buffer 1hPrimary Ab: O/N incubation at 4C, 1:1000Secondary Ab: IRDye 800CW Goat anti-Rabbit, 1:15000.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Brain tissues, human.

GAD65 Antibody Western Blot, validation (1:1000 dilution) in Brain tissues, human. (Cat no:20746-1-AP)

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