Anticorps Recombinant de lapin anti-GAD65

GAD65 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

250044B5

N° de cat : 85750-1-RR

Synonymes

GAD2, EC:4.1.1.15, GAD 65, GAD-65

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Galerie de données de validation

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  WB analysis of human cerebellum using 85750-1-RR

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 85750-1-RR (GAD2 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  WB analysis using 85750-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85750-1-RR (GAD2 antibody) at dilution of 1:300000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu de cervelet de souris, tissu cérébral de rat, tissu cérébral de souris, tissu de cervelet humain

Dilution recommandée

• Western Blot (WB): WB : 1:20000-1:100000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85750-1-RR cible GAD65 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)
• Masse moléculaire calculée: 65 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: NM_000818
• Symbole du gène: GAD65
• Identification du gène (NCBI): 2572
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GAD2, also named as GAD65, belongs to the group II decarboxylase family. GAD2 catalyzes the production of GABA. It is responsible for the synthesis of the essential neurotransmitter gamma-aminobutyric acid (GABA) from L-glutamic acid. GAD2 is expressed in nervous and endocrine systems and are thought to be involved in synaptic transmission and INS secretion. Autoantibodies against GAD2 may serve as markers for type I diabetes. Many individuals suffering from an adult onset disorder known as Stiff Person Syndrome (SPS) also express autoantibodies to GAD2. The antibody is specific to GAD2.

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