Anticorps Polyclonal de lapin anti-GAD65

GAD65 Polyclonal Antibody for WB, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 20746-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 20746-1-AP (same clone as 20746-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20746-1-AP (GAD65 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20746-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 20746-1-AP (same clone as 20746-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20746-1-AP (GAD65 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20746-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 20746-1-AP (same clone as 20746-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20746-1-AP (GAD65 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20746-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 20746-1-AP (same clone as 20746-1-PBS)

  IP result of anti-GAD65 (IP:20746-1-AP, 4ug; Detection:20746-1-AP 1:1000) with mouse brain tissue lysate 3440ug. This data was developed using the same antibody clone with 20746-1-PBS in a different storage buffer formulation.

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Informations sur le produit

20746-1-PBS cible GAD65 dans les applications de WB, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)
• Masse moléculaire calculée: 65 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: NM_000818
• Symbole du gène: GAD65
• Identification du gène (NCBI): 2572
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GAD2, also named as GAD65, belongs to the group II decarboxylase family. GAD2 catalyzes the production of GABA. It is responsible for the synthesis of the essential neurotransmitter gamma-aminobutyric acid (GABA) from L-glutamic acid. GAD2 is expressed in nervous and endocrine systems and are thought to be involved in synaptic transmission and INS secretion. Autoantibodies against GAD2 may serve as markers for type I diabetes. Many individuals suffering from an adult onset disorder known as Stiff Person Syndrome (SPS) also express autoantibodies to GAD2. The antibody is specific to GAD2.

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