Anticorps Recombinant de lapin anti-GADD34

GADD34 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

4K3

N° de cat : 81250-1-RR

Synonymes

PPP1R15A, Growth arrest and DNA damage-inducible protein GADD34, growth arrest and DNA damage-inducible protein 34, 4K3

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 81250-1-RR

  MG132 treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 81250-1-RR (GADD34 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 81250-1-RR

  MG132 treated HeLa cells were subjected to SDS PAGE followed by western blot with 81250-1-RR (GADD34 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 81250-1-RR

  MG132 treated HepG2 cells were subjected to SDS PAGE followed by western blot with 81250-1-RR (GADD34 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IF Staining of PC-3 using 81250-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using GADD34 antibody (81250-1-RR, Clone: 4K3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 81250-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81250-1-RR against Human GADD34 were performed. The affinity constant is 93.8 pM.

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Applications testées

• Résultats positifs en WB: MG132 treated HepG2 cells, cellules NIH/3T3 traitées au MG132
• Résultats positifs en IF/ICC: cellules PC-3,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

81250-1-RR cible GADD34 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: GADD34 Protéine recombinante Ag0578
• Nom complet: protein phosphatase 1, regulatory (inhibitor) subunit 15A
• Masse moléculaire calculée: 73 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC003067
• Symbole du gène: GADD34
• Identification du gène (NCBI): 23645
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GADD34, also named PPP1R15A, belongs to the PPP1R15 family. GADD34 can be triggered as a direct target of activating transcription factor4 (ATF4) under ER stress, it plays a pivotal role in the recovery of cells from shut-down of translation induced by ER stress. It recruits the serine/threonine-protein phosphatase (PP1) to dephosphorylate the translation initiation factor eIF2alpha, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress. GADD34 down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 via PP1. It may also promote apoptosis by inducing TP53 phosphorylation on 'Ser-15'. Starvation-induced expression of GADD34 reduced mTOR activity and induced autophagy in wild-type mice, but not in GADD34 KO mice. Molecular weight of GADD34 is 100 kDa confirmed in GADD34 KO mice, and Proteintech's GADD34 antibody 81250-1-RR primarily recongizes the 100 kDa band.

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