Anticorps Recombinant de lapin anti-GADD45GIP1

GADD45GIP1 Recombinant Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

240947D1

N° de cat : 83823-5-PBS

Synonymes

CRIF1, CR6-interacting factor 1, CKII beta-associating protein, CKBBP2, 39S ribosomal protein L59, mitochondrial

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Galerie de données de validation

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  WB analysis using 83823-5-RR (same clone as 83823-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83823-5-RR (GADD45GIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83823-5-PBS in a different storage buffer formulation.

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  IHC staining of human ovarian cancer using 83823-5-RR (same clone as 83823-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83823-5-RR (GADD45GIP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83823-5-PBS in a different storage buffer formulation.

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  IHC staining of human ovarian cancer using 83823-5-RR (same clone as 83823-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83823-5-RR (GADD45GIP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83823-5-PBS in a different storage buffer formulation.

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Informations sur le produit

83823-5-PBS cible GADD45GIP1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: GADD45GIP1 Protéine recombinante Ag9299
• Nom complet: growth arrest and DNA-damage-inducible, gamma interacting protein 1
• Masse moléculaire calculée: 222 aa, 25 kDa
• Poids moléculaire observé: 25-28 kDa
• Numéro d’acquisition GenBank: BC013027
• Symbole du gène: GADD45GIP1
• Identification du gène (NCBI): 90480
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Growth arrest and DNA-damage-inducible proteins-interacting protein 1(GADD45GIP1), also known as CRIF1, is newly identified de novo components in large subunit of mitoribosome. It is essential for the translation of mitochondrial oxidative phosphorylation (OXPHOS) polypeptides in mammalian mitochondria. The essential role of CRIF1 in mitochondrial synthesis and membrane integration of OXPHOS polypeptides was shown in brain-specific CRIF1-deficient mice, which exhibited profound OXPHOS failure and marked neurodegeneration. (PMID 22819524) In addition, GADD45GIP1 enhances the function of GADD45 in maintaining genome stability, DNA repair and cell cycle regulation by interacting with the GADD45 protein family. It's reported that GADD45GIP1 may be induced by p53, a process that is closely related to the cell's response to DNA damage.(PMID:28599472)

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