Anticorps Polyclonal de lapin anti-Galectin-3

• Phare
• Validé par KD/KO

Galectin-3 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications(46)

Avis (2)

N° de cat : 14979-1-AP

Synonymes

35 kDa lectin, Carbohydrate-binding protein 35, CBP 35, CBP35, Gal 3

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Galerie de données de validation

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  WB analysis of HeLa cells using 14979-1-AP

  WB result of Galectin 3 antibody (14979-1-AP, 1:1000) with si-Control and si-Galectin 3 transfected HeLa cells.

• 

  WB analysis using 14979-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14979-1-AP (Galectin-3 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat colon using 14979-1-AP

  rat colon tissue were subjected to SDS PAGE followed by western blot with 14979-1-AP (Galectin-3 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 14979-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14979-1-AP (Galectin-3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 14979-1-AP

  IP result of anti-Galectin-3 (IP:14979-1-AP, 3ug; Detection:14979-1-AP 1:500) with MCF-7 cells lysate 1600ug.

• 

  IHC staining of human thyroid cancer using 14979-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 14979-1-AP (Galectin-3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 14979-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 14979-1-AP (Galectin-3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 14979-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14979-1-AP (Galectin-3 antibody) at dilution of 1:8000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 14979-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Galectin-3 antibody (14979-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  FC experiment of HeLa using 14979-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug Galectin-3 Polyclonal antibody (14979-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2) (red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules MCF-7, cellules NIH/3T3, tissu cardiaque humain, tissu de côlon de rat
• Résultats positifs en IP: cellules MCF-7
• Résultats positifs en IHC: tissu de cancer de la thyroïde humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 26 publications below
• IHC: See 13 publications below
• IF: See 18 publications below
• IP: See 3 publications below

Informations sur le produit

14979-1-AP cible Galectin-3 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Galectin-3 Protéine recombinante Ag6891
• Nom complet: lectin, galactoside-binding, soluble, 3
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: 31 kDa
• Numéro d’acquisition GenBank: BC001120
• Symbole du gène: Galectin-3
• Identification du gène (NCBI): 3958
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-3, a member of the β-galactoside-binding proteins, contains one carbohydrate recognition domain (CRD) and a proline- and glycine-rich N-terminal domain through which is able to form oligomers (PMID: 14758078). Galectin-3 is widely expressed in many normal tissues and a variety of tumors. It is found intracellularly in nucleus and cytoplasm or secreted outside of cell, being present on the cell surface or in the extracellular space (PMID: 16478649). Galectin-3 is involved in various biological processes including cell growth, adhesion, differentiation, apoptosis, angiogenesis, immune response, neoplastic transformation and metastasis (PMID: 16478649; 14758078).

Publications

Species	Application	Title
human	IF	Nature; A phosphoinositide signalling pathway mediates rapid lysosomal repair; Authors - Jay Xiaojun Tan
mouse	WB	Nat Immunol; Heme drives hemolysis-induced susceptibility to infection via disruption of phagocyte functions.; Authors - Rui Martins
mouse	IF	Redox Biol; Cathepsin C from extracellular histone-induced M1 alveolar macrophages promotes NETosis during lung ischemia-reperfusion injury; Authors - Jing Yu
mouse	IF	Br J Pharmacol; Substitution of the SERCA2 Cys(674) reactive thiol accelerates atherosclerosis by inducing endoplasmic reticulum stress and inflammation; Authors - Hang Su
human	IF	J Neuroinflammation; Progranulin haploinsufficiency mediates cytoplasmic TDP-43 aggregation with lysosomal abnormalities in human microglia; Authors - Wonjae Sung
	WB	Acta Pharmacol Sin; Modified citrus pectin inhibits breast cancer development in mice by targeting tumor-associated macrophage survival and polarization in hypoxic microenvironment; Authors - Lei Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Víctor (Verified Customer) (09-18-2024)

The staining appears specific, although there is a bit of background, maybe due to the secondary antibody used. It is quite similar to the staining shown on the product's website

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Reactive astrocites

FH

Tom (Verified Customer) (07-07-2021)

HEK293 whole cell lysates on SDS-PAGE. Antibody incubated with membrane overnight in fridge. HRP secondary antibody used for detection.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293

Galectin-3 Antibody Western Blot validation (1:1000 dilution) in HEK293 (Cat no:14979-1-AP)

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