- Phare
- Validé par KD/KO
Anticorps Monoclonal anti-Galectin-3
Galectin-3 Monoclonal Antibody for FC, IF, IHC, WB, ELISA
Hôte / Isotype
Mouse / IgG2b
Réactivité testée
Humain, rat, souris
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugaison
Non conjugué
CloneNo.
1C1B2
N° de cat : 60207-1-Ig
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | cellules A431, cellules A549, cellules Caco-2, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules MCF-7, cellules T-47D, tissu placentaire humain |
| Résultats positifs en IHC | tissu de cancer de la thyroïde humain, tissu de côlon humain, tissu de tumeur ovarienne humain il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. |
| Résultats positifs en IF | cellules HeLa, cellules MCF-7, tissu de cancer de la thyroïde humain, tissu rénal de souris |
| Résultats positifs en cytométrie | cellules HeLa, |
Dilution recommandée
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochimie (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF) | IF : 1:400-1:1600 |
| Flow Cytometry (FC) | FC : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| KD/KO | See 2 publications below |
| WB | See 18 publications below |
| IHC | See 8 publications below |
| IF | See 14 publications below |
| IP | See 1 publications below |
Informations sur le produit
60207-1-Ig cible Galectin-3 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris
| Réactivité | Humain, rat, souris |
| Réactivité citée | rat, Humain, souris |
| Hôte / Isotype | Mouse / IgG2b |
| Clonalité | Monoclonal |
| Type | Anticorps |
| Immunogène | Galectin-3 Protéine recombinante Ag6753 |
| Nom complet | lectin, galactoside-binding, soluble, 3 |
| Masse moléculaire calculée | 26 kDa |
| Poids moléculaire observé | 31 kDa |
| Numéro d’acquisition GenBank | BC001120 |
| Symbole du gène | LGALS3 |
| Identification du gène (NCBI) | 3958 |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par protéine A |
| Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-3, a member of the β-galactoside-binding proteins, contains one carbohydrate recognition domain (CRD) and a proline- and glycine-rich N-terminal domain through which is able to form oligomers (PMID: 14758078). Galectin-3 is widely expressed in many normal tissues and a variety of tumors. It is found intracellularly in nucleus and cytoplasm or secreted outside of cell, being present on the cell surface or in the extracellular space (PMID: 16478649). Galectin-3 is involved in various biological processes including cell growth, adhesion, differentiation, apoptosis, angiogenesis, immune response, neoplastic transformation and metastasis (PMID: 16478649; 14758078).
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for Galectin-3 antibody 60207-1-Ig | Download protocol |
| IHC protocol for Galectin-3 antibody 60207-1-Ig | Download protocol |
| IF protocol for Galectin-3 antibody 60207-1-Ig | Download protocol |
| FC protocol for Galectin-3 antibody 60207-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Res CD248 Regulates Wnt Signaling in Pericytes to Promote Angiogenesis and Tumor Growth in Lung Cancer | ||
Circ. Res. Inhibition of KLF5-Myo9b-RhoA Pathway-Mediated Podosome Formation in Macrophages Ameliorates Abdominal Aortic Aneurysm. | ||
Acta Biomater Tumor cell membrane-based peptide delivery system targeting the tumor microenvironment for cancer immunotherapy and diagnosis. | ||
Front Immunol Single-cell RNA sequencing reveals a novel inhibitory effect of ApoA4 on NAFL mediated by liver-specific subsets of myeloid cells | ||
Theranostics Salvia miltiorrhiza-derived miRNAs suppress vascular remodeling through regulating OTUD7B/KLF4/NMHC IIA axis. | ||
Cell Rep Hepatocellular Carcinomas Originate Predominantly from Hepatocytes and Benign Lesions from Hepatic Progenitor Cells. |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Kenzo (Verified Customer) (08-29-2023) | I used this antibody in mouse kidney tissues and it worked well.
|
FH Muhammad (Verified Customer) (09-13-2022) | Work best in HUVECs
|
FH Noémie (Verified Customer) (10-20-2021) | I used this antibody for Western Blot against Gal3 in mamalian cells (U2OS), and did a validation by siRNA.
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