Anticorps Monoclonal anti-Galectin-3

• Phare
• Validé par KD/KO

Galectin-3 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(51)

CloneNo.

1C1B2

Avis (4)

N° de cat : 60207-1-Ig

Synonymes

Gal 3, CBP35, CBP 35, Carbohydrate-binding protein 35, 35 kDa lectin

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Galerie de données de validation

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  WB analysis using 60207-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60207-1-Ig (Galectin-3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa cells using 60207-1-Ig

  WB result of Galectin-3 specific antibody (60207-1-Ig, 1:1000) with si-control and si-Galectin-3 transfected HeLa cells.

• 

  WB analysis of HSC-T6 using 60207-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 60207-1-Ig (Galectin-3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human thyroid cancer using 60207-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60207-1-Ig (Galectin-3 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 60207-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60207-1-Ig (Galectin-3 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 60207-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60207-1-Ig (Galectin-3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 60207-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60207-1-Ig (Galectin-3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 60207-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60207-1-Ig (Galectin-3 antibody) at dilution of 1:15000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 60207-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60207-1-Ig (Galectin-3 antibody at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human thyroid cancer using 60207-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60207-1-Ig (Galectin-3 antibody at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary tumor using 60207-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60207-1-Ig (Galectin-3 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 60207-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60207-1-Ig (Galectin-3 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse kidney using 60207-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using Galectin-3 antibody (60207-1-Ig, Clone: 1C1B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse kidney using 60207-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using Galectin-3 antibody (60207-1-Ig, Clone: 1C1B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse kidney using 60207-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using Galectin-3 antibody (60207-1-Ig, Clone: 1C1B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human thyroid cancer using 60207-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human thyroid cancer tissue using 60207-1-Ig (Galectin-3 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HeLa using 60207-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Galectin-3 antibody (60207-1-Ig, Clone: 1C1B2 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of MCF-7 using 60207-1-Ig

  Immunofluorescent analysis of MCF-7 cells using 60207-1-Ig (Galectin-3 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG.

• 

  FC experiment of HeLa using 60207-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 0.8 ug Anti-Human Galectin-3 (60207-1-Ig, Clone:1C1B2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.8 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules A549, cellules Caco-2, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules MCF-7, cellules T-47D, tissu placentaire humain
• Résultats positifs en IHC: tissu de cancer de la thyroïde humain, tissu de côlon humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu rénal de souris, tissu de cancer de la thyroïde humain
• Résultats positifs en IF/ICC: cellules HeLa, cellules MCF-7, tissu de cancer de la thyroïde humain, tissu rénal de souris
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 31 publications below
• IHC: See 10 publications below
• IF: See 26 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Informations sur le produit

60207-1-Ig cible Galectin-3 dans les applications de WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Galectin-3 Protéine recombinante Ag6753
• Nom complet: lectin, galactoside-binding, soluble, 3
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: 31 kDa
• Numéro d’acquisition GenBank: BC001120
• Symbole du gène: Galectin-3
• Identification du gène (NCBI): 3958
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-3, a member of the β-galactoside-binding proteins, contains one carbohydrate recognition domain (CRD) and a proline- and glycine-rich N-terminal domain through which is able to form oligomers (PMID: 14758078). Galectin-3 is widely expressed in many normal tissues and a variety of tumors. It is found intracellularly in nucleus and cytoplasm or secreted outside of cell, being present on the cell surface or in the extracellular space (PMID: 16478649). Galectin-3 is involved in various biological processes including cell growth, adhesion, differentiation, apoptosis, angiogenesis, immune response, neoplastic transformation and metastasis (PMID: 16478649; 14758078).

Publications

Species	Application	Title
mouse	IF	Nat Commun; Surplus fatty acid synthesis increases oxidative stress in adipocytes and lnduces lipodystrophy; Authors - Li Weng
rat	WB,IF	Cell Death Differ; NLRX1 mediated impaired microglial phagocytosis of NETs in cerebral ischemia and reperfusion injury; Authors - Jialing Peng
mouse	WB,IF,IP	Cancer Res; CD248 Regulates Wnt Signaling in Pericytes to Promote Angiogenesis and Tumor Growth in Lung Cancer; Authors - Chia-Lun Hong
human	WB,IF	Autophagy; SMURF1 controls the PPP3/calcineurin complex and TFEB at a regulatory node for lysosomal biogenesis; Authors - Qin Xia
mouse	IF	Circ. Res.; Inhibition of KLF5-Myo9b-RhoA Pathway-Mediated Podosome Formation in Macrophages Ameliorates Abdominal Aortic Aneurysm.; Authors - Dong Ma
human	WB	Acta Biomater; Tumor cell membrane-based peptide delivery system targeting the tumor microenvironment for cancer immunotherapy and diagnosis.; Authors - Xiangzhou Meng

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Marco (Verified Customer) (07-09-2024)

works perfectly fine

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000/1:500
• Cell Tissue Type: NRVCMs

FH

Kenzo (Verified Customer) (08-29-2023)

I used this antibody in mouse kidney tissues and it worked well.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Mouse kidney

FH

Muhammad (Verified Customer) (09-13-2022)

Work best in HUVECs

• Applications: Western Blot
• Cell Tissue Type: HUVEC

FH

Noémie (Verified Customer) (10-20-2021)

I used this antibody for Western Blot against Gal3 in mamalian cells (U2OS), and did a validation by siRNA.

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: U2OS cells

Galectin-3 Antibody Western Blot validation (1/1000 dilution) in U2OS cells (Cat no:60207-1-Ig)

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