Anticorps Recombinant de lapin anti-GALNT2

GALNT2 Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240723G10

N° de cat : 83703-2-PBS

Synonymes

240723G10, EC:2.4.1.41, GalNAc T2, GalNAc-T2, Polypeptide N-acetylgalactosaminyltransferase 2

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Galerie de données de validation

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  WB analysis using 83703-2-RR (same clone as 83703-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83703-2-RR (GALNT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83703-2-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 83703-2-RR (same clone as 83703-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83703-2-RR (GALNT2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83703-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00666-1

  Cytometric bead array standard curve of MP00666-1, GALNT2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83703-1-PBS. Detection antibody: 83703-2-PBS. Standard: Ag11523. Range: 1.563-100 ng/mL.

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  Affinity and Kinetic Characterization of 83703-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83703-2-RR against Human GALNT2 were performed. The affinity constant is 2.04 nM.

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Informations sur le produit

83703-2-PBS cible GALNT2 dans les applications de WB, IHC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: GALNT2 Protéine recombinante Ag11523
• Nom complet: UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 2 (GalNAc-T2)
• Masse moléculaire calculée: 571 aa, 65 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: BC041120
• Symbole du gène: GALNT2
• Identification du gène (NCBI): 2590
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GALNT2, a GalNAc-transferase involved in O-linked glycosylation, modulates the risk of atherogenic dyslipidemia, a specific hallmark of insulin resistance and acts as a positive modulator of insulin signaling in human liver cells by down-regulating ENPP1. GALNT2 gene encodes GalNAc-T2 as a regulator of high-density lipoprotein cholesterol (HDL-C) metabolism (PMID: 31040393, 27508872). Western blot analysis detected GALNT2 at an apparent molecular mass of 64 kDa.

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