Anticorps Polyclonal de lapin anti-GALNT3

GALNT3 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 16716-1-AP

Synonymes

GalNAc T3, GALNT3, HFTC, HHS, pp GaNTase 3

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Galerie de données de validation

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  WB analysis of PC-3 using 16716-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16716-1-AP (GALNT3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 16716-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16716-1-AP (GALNT3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of DU 145 using 16716-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 16716-1-AP (GALNT3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16716-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16716-1-AP (GALNT3 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

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  WB analysis of mouse liver using 16716-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16716-1-AP (GALNT3 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of Transfected HEK-293 using 16716-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 16716-1-AP (GALNT3 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 16716-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16716-1-AP (GALNT3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 16716-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16716-1-AP (GALNT3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules PC-3, cellules DU 145, cellules HEK-293 transfectées, cellules HeLa, tissu hépatique de souris, tissu testiculaire de souris
• Résultats positifs en IHC: tissu rénal de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 2 publications below

Informations sur le produit

16716-1-AP cible GALNT3 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GALNT3 Protéine recombinante Ag10140
• Nom complet: UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 3 (GalNAc-T3)
• Masse moléculaire calculée: 633aa,73 kDa; 192aa,22 kDa
• Poids moléculaire observé: 70-75 kDa
• Numéro d’acquisition GenBank: BC056246
• Symbole du gène: GALNT3
• Identification du gène (NCBI): 2591
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Cell Cycle; LINC02535/miR-30a-5p/GALNT3 axis contributes to lung adenocarcinoma progression via the NF- κ B signaling pathway.; Authors - Yue Li
mouse	IHC	Bone; Growth-related skeletal changes and alterations in phosphate metabolism.; Authors - Toshimi Michigami
human	WB	J Exp Clin Cancer Res; LINC01296/miR-26a/GALNT3 axis contributes to colorectal cancer progression by regulating O-glycosylated MUC1 via PI3K/AKT pathway.; Authors - Bing Liu
human	IHC	Hum Cell; GalNAc-T3 and MUC1, a combined predictor of prognosis and recurrence in solitary pulmonary adenocarcinoma initially diagnosed as malignant solitary pulmonary nodule (≤ 3 cm).; Authors - Qiang Xie
rat	WB	Exp Ther Med; PMID: 29805509; Authors - Yanshao Liu
mouse	WB	Environ Toxicol; Cisplatin induces acute kidney injury by downregulating miR-30e-5p that targets Galnt3 to activate the AMPK signaling pathway; Authors - Weipu Mao