Anticorps Polyclonal de lapin anti-GALNTL2

GALNTL2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 16900-1-AP

Synonymes

EC:2.4.1.41, GalNAc-T-like protein 2, GALNT15, GALNT7, PIH5

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Galerie de données de validation

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  WB analysis of HEK-293 using 16900-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16900-1-AP (GALNTL2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 16900-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16900-1-AP (GALNTL2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse small intestine using 16900-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 16900-1-AP (GALNTL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 16900-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 16900-1-AP (GALNTL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 16900-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16900-1-AP (GALNTL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 16900-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16900-1-AP (GALNTL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 16900-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16900-1-AP (GALNTL2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 16900-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16900-1-AP (GALNTL2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 16900-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16900-1-AP (GALNTL2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 16900-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16900-1-AP (GALNTL2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 16900-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16900-1-AP (GALNTL2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 16900-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16900-1-AP (GALNTL2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 16900-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16900-1-AP (GALNTL2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 16900-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16900-1-AP (GALNTL2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 16900-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16900-1-AP (GALNTL2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 16900-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16900-1-AP (GALNTL2 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, tissu hépatique humain
• Résultats positifs en IHC: tissu d'intestin grêle de souris, tissu cérébral humain, tissu ovarien humain, tissu placentaire humain, tissu pulmonaire humain, tissu rénal humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

16900-1-AP cible GALNTL2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GALNTL2 Protéine recombinante Ag10434
• Nom complet: UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase-like 2
• Masse moléculaire calculée: 639 aa, 73 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: BC014789
• Symbole du gène: GALNTL2
• Identification du gène (NCBI): 117248
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GALNTL2, also known as GALNT15, is a member of the polypeptide ppGalNAc-T family. It catalyzes the initial step in O-linked oligosaccharide biosynthesis by transferring an N-acetyl-D-galactosamine residue to serine or threonine residues on protein receptors. Though it shows weaker activity compared to GALNT2, it can transfer up to seven GalNAc residues to the Muc5AC peptide, suggesting cooperative action with other GALNT proteins. GALNTL2 is widely expressed, with high expression in tissues like the small intestine, placenta, spleen, cerebral cortex, and ovary.

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