Anticorps Polyclonal de lapin anti-GAMT

GAMT Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(11)

Avis (1)

N° de cat : 10880-1-AP

Synonymes

GAMT, PIG2, TP53I2

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Galerie de données de validation

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  WB analysis using 10880-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10880-1-AP (GAMT antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 10880-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 10880-1-AP (GAMT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 10880-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10880-1-AP (GAMT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 10880-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10880-1-AP (GAMT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 10880-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10880-1-AP (GAMT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10880-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10880-1-AP (GAMT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10880-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10880-1-AP (GAMT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10880-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10880-1-AP (GAMT antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 10880-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10880-1-AP (GAMT antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 10880-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 10880-1-AP (GAMT antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 10880-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 10880-1-AP (GAMT antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HepG2, cellules SH-SY5Y, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IHC: tissu hépatique humain, tissu de cancer de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

10880-1-AP cible GAMT dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GAMT Protéine recombinante Ag1327
• Nom complet: guanidinoacetate N-methyltransferase
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: 26 kDa
• Numéro d’acquisition GenBank: BC016760
• Symbole du gène: GAMT
• Identification du gène (NCBI): 2593
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GAMT converts the latter to creatine with S-adenosylmethionine as the methyl donor. GAMT was induced in human and mouse cells in response to genotoxic and metabolic stress. GAMT was required for p53-dependent upregulation of creatine biosynthesis, fatty acid oxidation, and apoptosis in response to glucose deprivation.

Publications

Species	Application	Title
mouse	WB	J Agric Food Chem; Artepillin C, a key component of Brazilian propolis, induces thermogenesis in inguinal white adipose tissue of mice through a creatine metabolism-related thermogenic pathway.; Authors - Sho Nishikawa
mouse	WB	Toxicol Appl Pharmacol; Cytochalasin Q exerts anti-melanoma effect by inhibiting creatine kinase B.; Authors - Yi Lu
rat	WB	Ann Transl Med; Transcriptomics combined with metabolomics analysis of the mechanism of agmatine in the treatment of septic liver injury.; Authors - Ling Huang
mouse	WB,IF	Brain Res; GATM and GAMT synthesize creatine locally throughout the mammalian body and within oligodendrocytes of the brain.; Authors - Steven Andrew Baker
zebrafish		J Fish Biol; Expression patterns of the creatine metabolism-related molecules AGAT, GAMT and CT1 in adult zebrafish Danio rerio.; Authors - Wang L L
mouse	WB	Redox Biol; Unbalanced redox status network as an early pathological event in congenital cataracts; Authors - Eloy Bejarano

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Werner (Verified Customer) (11-28-2017)

13% SDS-PAGE, 10 ug total liver lysate loaded, overnight incubation with primary antibody at 4°C

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Mouse liver

GAMT Antibody Western Blot, validation (1:2000 dilution) in Mouse liver (Cat no:10880-1-AP)

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