Anticorps Monoclonal anti-GAMT

GAMT Monoclonal Antibody for WB,ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

2

CloneNo.

1E7B11

N° de cat : 66322-1-Ig

Synonymes

GAMT, PIG2, TP53I2

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Galerie de données de validation

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  WB analysis of fetal human brain using 66322-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66322-1-Ig (GAMT Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 66322-1-Ig

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 66322-1-Ig (GAMT Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 66322-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66322-1-Ig (GAMT Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig liver using 66322-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66322-1-Ig (GAMT Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu cérébral humain fœtal, tissu hépatique de porc, tissu hépatique de rat, tissu hépatique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

66322-1-Ig cible GAMT dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GAMT Protéine recombinante Ag16778
• Nom complet: guanidinoacetate N-methyltransferase
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: 26 kDa
• Numéro d’acquisition GenBank: BC016760
• Symbole du gène: GAMT
• Identification du gène (NCBI): 2593
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GAMT converts the latter to creatine with S-adenosylmethionine as the methyl donor. GAMT was induced in human and mouse cells in response to genotoxic and metabolic stress. GAMT was required for p53-dependent upregulation of creatine biosynthesis, fatty acid oxidation, and apoptosis in response to glucose deprivation.

Publications

Species	Application	Title
mouse	WB	Food Funct; The protective effect of polyunsaturated fatty acid intake during pregnancy against embryotoxicity of sodium valproate in mice; Authors - Kelei Li
human	WB	BMC Cardiovasc Disord; PMID: 31888640; Authors - Zhuyin Jia