Anticorps Recombinant de lapin anti-GANAB

• Phare
• Validé par KD/KO

GANAB Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240279B7

N° de cat : 83324-1-PBS

Synonymes

Glucosidase II subunit alpha, G2AN, EC:3.2.1.207, Alpha-glucosidase 2, Alpha glucosidase 2

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Galerie de données de validation

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  WB analysis using 83324-1-RR (same clone as 83324-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83324-1-RR (GANAB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83324-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 83324-1-RR (same clone as 83324-1-PBS)

  WB result of GANAB antibody (83324-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GANAB transfected HeLa cells. This data was developed using the same antibody clone with 83324-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 83324-1-RR (same clone as 83324-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83324-1-RR (GANAB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83324-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83324-1-RR (same clone as 83324-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GANAB antibody (83324-1-RR, Clone: 240279B7 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83324-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83324-1-RR (same clone as 83324-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug GANAB Recombinant antibody (83324-1-RR, Clone:240279B7)(red) or Isotype Controland (blue) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83324-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00350-1

  Cytometric bead array standard curve of MP00350-1, GANAB Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83324-1-PBS. Detection antibody: 83324-2-PBS. Standard: Ag30417. Range: 1.25-40 ng/mL.

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  Affinity and Kinetic Characterization of 83324-1-PBS

  Biolayer interferometry (BLl) kinetic assay of 83324-1-PBS against Human GANAB was performed. The affinity constant is 0.16 nM.

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  Affinity and Kinetic Characterization of 83324-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83324-1-RR against Human GANAB were performed. The affinity constant is 0.186 nM.

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Informations sur le produit

83324-1-PBS cible GANAB dans les applications de WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: GANAB Protéine recombinante Ag30417
• Nom complet: glucosidase, alpha; neutral AB
• Masse moléculaire calculée: 944aa,107 kDa; 488aa,55 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: BC017435
• Symbole du gène: GANAB
• Identification du gène (NCBI): 23193
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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