Anticorps Monoclonal anti-GAPDH

• Phare
• Validé par KD/KO

GAPDH Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, levure, poisson-zèbre, porc, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

1E6D9

N° de cat : 60004-1-PBS

Synonymes

1E6D9

---

Galerie de données de validation

• 

  WB analysis using 60004-1-Ig (same clone as 60004-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60004-1-Ig (GAPDH antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 60004-1-Ig (same clone as 60004-1-PBS)

  Western blot analysis of GAPDH in various tissues and cell lines using Proteintech antibody 60004-1-Ig at a dilution of 1:10000. This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 60004-1-Ig (same clone as 60004-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60004-1-Ig (GAPDH antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 60004-1-Ig (same clone as 60004-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60004-1-Ig (GAPDH antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

• 

  WB analysis of arabidopsis whole plant using 60004-1-Ig (same clone as 60004-1-PBS)

  arabidopsis, rice, wheat, corn whole plant tissue were subjected to SDS PAGE followed by western blot with 60004-1-Ig (GAPDH Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 60004-1-Ig (same clone as 60004-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60004-1-Ig (GAPDH antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

• 

  WB analysis of whole Nematode using 60004-1-Ig (same clone as 60004-1-PBS)

  whole Nematode tissue were subjected to SDS PAGE followed by western blot with 60004-1-Ig (GAPDH Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

• 

  WB analysis of soybean whole plant using 60004-1-Ig (same clone as 60004-1-PBS)

  soybean whole plant tissue were subjected to SDS PAGE followed by western blot with 60004-1-Ig (GAPDH Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

• 

  WB analysis of zebrafish using 60004-1-Ig (same clone as 60004-1-PBS)

  zebrafish tissue were subjected to SDS PAGE followed by western blot with 60004-1-Ig (GAPDH antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

• 

  WB analysis of whole yeast using 60004-1-Ig (same clone as 60004-1-PBS)

  whole yeast cells were subjected to SDS PAGE followed by western blot with 60004-1-Ig (GAPDH antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 60004-1-Ig (same clone as 60004-1-PBS)

  Western blot of Hela cell with anti-GAPDH (60004-1-Ig) at various dilutions. This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa Cells using 60004-1-Ig (same clone as 60004-1-PBS)

  IP result of anti-GAPDH (60004-1-Ig for IP and Detection) with HeLa cell lysate. This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 60004-1-Ig (same clone as 60004-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60004-1-Ig (GAPDH antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 60004-1-Ig (same clone as 60004-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60004-1-Ig (GAPDH antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 60004-1-Ig (same clone as 60004-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GAPDH antibody (60004-1-Ig, Clone: 1E6D9 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 60004-1-Ig (same clone as 60004-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human GAPDH (60004-1-Ig, Clone:1E6D9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

---

Applications publiées

• WB: See 1 publications below

Informations sur le produit

60004-1-PBS cible GAPDH dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, levure, poisson-zèbre, porc, rat, souris

• Réactivité: Humain, levure, poisson-zèbre, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GAPDH Protéine recombinante Ag0766
• Nom complet: glyceraldehyde-3-phosphate dehydrogenase
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 36 kDa
• Numéro d’acquisition GenBank: BC004109
• Symbole du gène: GAPDH
• Identification du gène (NCBI): 2597
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

What is the molecular weight of GAPDH?

The molecular weight of GAPDH is 36 kD.

What is GAPDH?

GAPDH stands for glyceraldehyde-3-phosphate dehydrogenase and is often referred to as a "housekeeping" 

protein. It is recognized as an important enzyme involved in metabolic pathways, aiding in the glycolytic 

production of ATP and pyruvate (PMID: 21895736).

What is the function of GAPDH?

GAPDH is responsible for catalyzing the phosphorylation of glyceraldehyde-3-phosphate into D-glycerate-1,3-biphosphate. While this function of GAPDH is considered its primary function, GAPDH is also involved, even 

critical, in other cell functions, some of which are still being studied. Recent studies have suggested that GAPDH 

is a key factor in regulating apoptosis, translocating to the nucleus under certain stressful conditions and acting 

as a signaling factor in oxidative stress-induced apoptosis. Other studies indicate that GAPDH plays a role in 

transporting vesicles from the endoplasmic reticulum to the Golgi body (PMIDs: 21895736, 12829261, 25859407, 

11035021).

Where in the cell is GAPDH localized?

GAPDH is expressed primarily in the cytosolic and membrane regions, with minimal expression in the nucleus 

(PMIDs: 21895736, 12829261).

Is there a reason to use an antibody targeting GAPDH if my research is not concerned with glycolysis or nuclear 

functions?

Yes! Because GAPDH is often highly expressed throughout different tissues and cell types, GAPDH expression is 

useful as a control for Western blots and RT-PCR.

What are the known protein-protein interactions of GAPDH?

GAPDH interacts with other proteins to regulate nuclear translocation and GAPDH catalytic activity. Some of 

these proteins include TPPP, SIAH1, CHP1, RILPL1/GOSPEL and FKBP6 (PMIDs: 21895736, 25859407).

Note: For murine tissue samples, conjugated mouse antibody HRP-60004 and rabbit antibody 10494-1-AP are preferable.

Publications

Species	Application	Title
	WB	Mol Cell Biochem; PSAT1 promotes the progression of colorectal cancer by regulating Hippo-YAP/TAZ-ID1 axis via AMOT; Authors - Minshan Tang

{{ptg:RelatedPrimaryAntibodies}}