Anticorps Recombinant de lapin anti-GAPDH
GAPDH Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, rat, souris
Applications
WB, IF/ICC, Cytometric bead array, Indirect ELISA
Conjugaison
Non conjugué
CloneNo.
1H18
N° de cat : 81640-5-PBS
Synonymes
Galerie de données de validation
Informations sur le produit
81640-5-PBS cible GAPDH dans les applications de WB, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris
Réactivité | Humain, rat, souris |
Hôte / Isotype | Lapin / IgG |
Clonalité | Recombinant |
Type | Anticorps |
Immunogène | GAPDH Protéine recombinante Ag0766 |
Nom complet | glyceraldehyde-3-phosphate dehydrogenase |
Masse moléculaire calculée | 36 kDa |
Poids moléculaire observé | 36 kDa |
Numéro d’acquisition GenBank | BC004109 |
Symbole du gène | GAPDH |
Identification du gène (NCBI) | 2597 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par protéine A |
Tampon de stockage | PBS only |
Conditions de stockage | Store at -80°C. 20ul contiennent 0,1% de BSA. |
Informations générales
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.