Anticorps Recombinant de lapin anti-GAPDH (Human Specific)

GAPDH (Human Specific) Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2G21

N° de cat : 80570-1-PBS

Synonymes

GAPDH, 2G21

---

Galerie de données de validation

• 

  WB analysis using 80570-1-RR (same clone as 80570-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80570-1-RR (GAPDH antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80570-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 80570-1-RR (same clone as 80570-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80570-1-RR (GAPDH (Human Specific) antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80570-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 80570-1-RR (same clone as 80570-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80570-1-RR (GAPDH (Human Specific) antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80570-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 80570-1-RR (same clone as 80570-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 80570-1-RR (GAPDH (Human Specific) antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80570-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 80570-1-RR (same clone as 80570-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 80570-1-RR (GAPDH (Human Specific) antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80570-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 80570-1-RR (same clone as 80570-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GAPDH (Human Specific) antibody (80570-1-RR, Clone: 2G21 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80570-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 80570-1-RR (same clone as 80570-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GAPDH (Human Specific) antibody (80570-1-RR, Clone: 2G21 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80570-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

80570-1-PBS cible GAPDH (Human Specific) dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: GAPDH (Human Specific) Protéine recombinante Ag0766
• Nom complet: glyceraldehyde-3-phosphate dehydrogenase
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 36 kDa
• Numéro d’acquisition GenBank: BC004109
• Symbole du gène: GAPDH
• Identification du gène (NCBI): 2597
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types. For murine tissue samples, conjugated mouse antibody HRP-60004 and rabbit antibody 10494-1-AP are preferable. 80570-1-RR is human specific.

{{ptg:RelatedPrimaryAntibodies}}