Anticorps Recombinant de lapin anti-GAR1

GAR1 Recombinant Antibody for IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240537C11

N° de cat : 83577-1-PBS

Synonymes

240537C11, NOLA1, snoRNP protein GAR1

---

Galerie de données de validation

• 

  IHC staining of human skin cancer using 83577-1-RR (same clone as 83577-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 83577-1-RR (GAR1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83577-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 83577-1-RR (same clone as 83577-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 83577-1-RR (GAR1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83577-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 83577-1-RR (same clone as 83577-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GAR1 antibody (83577-1-RR, Clone: 240537C11 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83577-1-PBS in a different storage buffer formulation.

• 

  FC experiment of K562 using 83577-1-RR (same clone as 83577-1-PBS)

  1x10^6 K562 were intracellularly stained with 0.25 ug GAR1 Recombinant antibody (83577-1-RR, Clone:240537C11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83577-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP00568-1

  Cytometric bead array standard curve of MP00568-1, GAR1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83577-2-PBS. Detection antibody: 83577-1-PBS. Standard: Ag2282. Range: 0.781-100 ng/mL.

• 

  Cytometric bead array standard curve of MP00568-2

  Cytometric bead array standard curve of MP00568-2, GAR1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83577-3-PBS. Detection antibody: 83577-1-PBS. Standard: Ag2282. Range: 0.781-100 ng/mL.

• 

  Affinity and Kinetic Characterization of 83577-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83577-1-RR against Human GAR1 were performed. The affinity constant is below 1 pM.

---

Informations sur le produit

83577-1-PBS cible GAR1 dans les applications de IHC, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: GAR1 Protéine recombinante Ag2282
• Nom complet: GAR1 ribonucleoprotein homolog (yeast)
• Masse moléculaire calculée: 217 aa, 22 kDa
• Numéro d’acquisition GenBank: BC003413
• Symbole du gène: GAR1
• Identification du gène (NCBI): 54433
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GAR1, other named NOLA1, is a subunit of H/ACA and telomerase. Thought is not required for H/ACA protein assembly, GAR1 is necessary for ribosome biogenesis and telomere. H/ACA involves in class specify the sites of uridine-to-pseudouridine. It contains a core domain that flanked by glycine- and arginine-rich(GAR) domains. GAR1 also required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transciptase(TERT) holoenzyme.

{{ptg:RelatedPrimaryAntibodies}}