Anticorps Recombinant de lapin anti-GARNL1

• Phare
• Validé par KD/KO

GARNL1 Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230165F11

N° de cat : 82971-1-PBS

Synonymes

Tuberin like protein 1, RALGAPA1, p240, KIAA0884, GRIPE

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Galerie de données de validation

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  WB analysis using 82971-1-RR (same clone as 82971-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82971-1-RR (GARNL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82971-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 82971-1-RR (same clone as 82971-1-PBS)

  WB result of GARNL1 antibody (82971-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GARNL1 transfected HEK-293 cells. This data was developed using the same antibody clone with 82971-1-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 82971-1-RR (same clone as 82971-1-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug GARNL1 Recombinant antibody (82971-1-RR, Clone:230165F11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82971-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82971-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82971-1-RR against Human GARNL1 were performed. The affinity constant is 99.6 pM.

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Informations sur le produit

82971-1-PBS cible GARNL1 dans les applications de WB, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: GARNL1 Protéine recombinante Ag31518
• Nom complet: GTPase activating Rap/RanGAP domain-like 1
• Masse moléculaire calculée: 230KD
• Poids moléculaire observé: 260 kDa
• Numéro d’acquisition GenBank: NM_014990.3
• Symbole du gène: GARNL1
• Identification du gène (NCBI): 253959
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GARNL1/RalGAPα1, a major α subunit of the Ral-GTPase activating protein in skeletal muscle, is a protein whose phosphorylation and binding to the regulatory 14-3-3 proteins is stimulated by insulin and also by muscle contraction (PMID:24768767).

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