Anticorps Polyclonal de lapin anti-GARS

• Phare
• Validé par KD/KO

GARS Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Conjugaison

Non conjugué

Publications(10)

N° de cat : 15831-1-AP

Synonymes

GARS1, EC:6.1.1.14, EC:2.7.7.-, DSMAV, CMT2D

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Galerie de données de validation

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  WB analysis of human brain using 15831-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15831-1-AP (GARS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15831-1-AP

  WB result of GARS antibody (15831-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GARS transfected HepG2 cells.

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  WB analysis of HeLa using 15831-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15831-1-AP (GARS Antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 15831-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 15831-1-AP (GARS Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15831-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15831-1-AP (GARS Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 15831-1-AP

  IP result of anti-GARS (IP:15831-1-AP, 4ug; Detection:15831-1-AP 1:500) with mouse liver tissue lysate 4000ug.

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  IHC staining of human skin cancer using 15831-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 15831-1-AP (GARS Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse brain using 15831-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15831-1-AP (GARS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 15831-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 15831-1-AP (GARS Antibody) at dilution of 1:200 (under 10x lens).

• 

  IF Staining of HepG2 using 15831-1-AP

  Immunofluorescent analysis of HepG2 cells, using GARS antibody 15831-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, cellules HeLa, cellules HepG2, cellules HT-1080
• Résultats positifs en IP: tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer de la peau humain, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• CoIP: See 2 publications below
• RIP: See 1 publications below

Informations sur le produit

15831-1-AP cible GARS dans les applications de WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Drosophile, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GARS Protéine recombinante Ag8322
• Nom complet: glycyl-tRNA synthetase
• Masse moléculaire calculée: 739 aa, 83 kDa
• Poids moléculaire observé: 75-80 kDa
• Numéro d’acquisition GenBank: BC007755
• Symbole du gène: GARS
• Identification du gène (NCBI): 2617
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GARS(glycinamide ribonucleotide synthetase) perform an essential function in protein synthesis by catalyzing the esterification of an amino acid to its cognate tRNA.GARS, however, is a ubiquitously expressed α2 homodimer responsible for charging tRNA molecules with glycine, this enzyme is essential for protein synthesis in all cells and tissues(PMID:17035524 ).It can exist as a oligomer with 250KDa(PMID:22504945).

Publications

Species	Application	Title
human,mouse	CoIP	Cell; Diverse CMT2 neuropathies are linked to aberrant G3BP interactions in stress granules; Authors - Qinqin Cui
human	WB	J Hematol Oncol; Integrated proteogenomic characterization of urothelial carcinoma of the bladder.; Authors - Ning Xu
mouse	WB	Aging Cell; SIRT2-knockdown rescues GARS-induced Charcot-Marie-Tooth neuropathy.; Authors - Yingying Zhao
mouse	WB,IF	Brain; HDAC6 is a therapeutic target in mutant GARS-induced Charcot-Marie-Tooth disease.; Authors - Veronick Benoy ; KD Validated
Drosophila	WB	Nat Commun; Impaired protein translation in Drosophila models for Charcot-Marie-Tooth neuropathy caused by mutant tRNA synthetases.; Authors - Sven Niehues
human	WB,RIP	EMBO Rep; The tRNA-like small noncoding RNA mascRNA promotes global protein translation.; Authors - Xinping Lu ; KD Validated

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