Anticorps Monoclonal anti-GARS

• Phare
• Validé par KD/KO

GARS Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2H9D3

N° de cat : 67893-1-PBS

Synonymes

AP 4 A synthetase, CMT2D, DSMAV, GARS, Glycine tRNA ligase, glycyl tRNA synthetase, GlyRS, HMN5, SMAD1

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Galerie de données de validation

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  WB analysis using 67893-1-Ig (same clone as 67893-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67893-1-Ig (GARS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67893-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 67893-1-Ig (same clone as 67893-1-PBS)

  WB result of GARS antibody (67893-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GARS transfected HepG2 cells. This data was developed using the same antibody clone with 67893-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 67893-1-Ig (same clone as 67893-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 67893-1-Ig (GARS antibody) at dilution of 1:2000 (under 40x lens). This data was developed using the same antibody clone with 67893-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 67893-1-Ig (same clone as 67893-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 67893-1-Ig (GARS antibody) at dilution of 1:2000 (under 40x lens). This data was developed using the same antibody clone with 67893-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 67893-1-Ig (same clone as 67893-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using GARS antibody (67893-1-Ig, Clone: 2H9D3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67893-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67893-1-PBS cible GARS dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GARS Protéine recombinante Ag8432
• Nom complet: glycyl-tRNA synthetase
• Masse moléculaire calculée: 739 aa, 83 kDa
• Poids moléculaire observé: 75-80 kDa
• Numéro d’acquisition GenBank: BC007755
• Symbole du gène: GARS
• Identification du gène (NCBI): 2617
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Glycyl-tRNA synthetase 1 (GARS, GeneID:2617) is one of the aminoacyl-tRNA synthetases. It is essential for protein synthesis in all cells and tissues (PMID: 17035524) and charges tRNA molecules with glycine by catalyzing the ATP-dependent ligation of glycine to the 3'-end of it's cognate tRNA via the formation of an aminoacyl-adenylate intermediate (Gly-AMP) (PMID: 17544401, PMID: 28675565, PMID: 24898252) . GARS is located in cytoplasm, but also can be secreted from cells and as an extracellular protein which may influence cell signaling. Mutation of GARS causes peripheral neuropathies such as CMT2D, SMA (PMID: 26503042, PMID: 32181591) , or associates with cancer. Two transcript variants encoding different isoforms have been found for this gene. The molecular weight of isoform2 is about 78 kDa.

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