Anticorps Polyclonal de lapin anti-GART

GART Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

7

Avis (1)

N° de cat : 13659-1-AP

Synonymes

AIR synthase, AIRS, GAR transformylase, GARS, GART, GARTF, PAIS, PGFT, PRGS

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Galerie de données de validation

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  WB analysis using 13659-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 13659-1-AP (GART antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 13659-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13659-1-AP (GART antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13659-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13659-1-AP (GART antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 13659-1-AP

  IP Result of anti-GART (IP:13659-1-AP, 4ug; Detection:13659-1-AP 1:500) with HepG2 cells lysate 1600ug.

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  IHC staining of human cervical cancer using 13659-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13659-1-AP (GART antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 13659-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13659-1-AP (GART antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 13659-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 13659-1-AP (GART antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HeLa, cellules HepG2
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

13659-1-AP cible GART dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GART Protéine recombinante Ag4555
• Nom complet: phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase
• Masse moléculaire calculée: 1010 aa, 108 kDa
• Poids moléculaire observé: 108 kDa, 46 kDa
• Numéro d’acquisition GenBank: BC038958
• Symbole du gène: GART
• Identification du gène (NCBI): 2618
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GART is a trifunctional polypeptide which has phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase activity that is required for de novo purine biosynthesis. GART has 2 isoforms with molecular mass of 108 kDa and 46 kDa due to alternative splicing.

Publications

Species	Application	Title
		Science; mTORC1 induces purine synthesis through control of the mitochondrial tetrahydrofolate cycle.; Authors - Issam Ben-Sahra
human	WB	Mol Cell; ERK2 Phosphorylates PFAS to Mediate Posttranslational Control of De Novo Purine Synthesis.; Authors - Eunus S Ali
mouse	WB	Nat Commun; Purine nucleotide depletion prompts cell migration by stimulating the serine synthesis pathway.; Authors - Mona Hoseini Soflaee
human	WB	EMBO J; UHMK1 promotes gastric cancer progression through reprogramming nucleotide metabolism.; Authors - Xing Feng
human	WB	Cell Rep; Purine Nucleotide Availability Regulates mTORC1 Activity through the Rheb GTPase.; Authors - Natasha Emmanuel
human	WB	Nat Metab; Mitochondrial NADP+ is essential for proline biosynthesis during cell growth.; Authors - Diem H Tran

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hua (Verified Customer) (08-26-2019)

Works well.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HepG2, mouse liver, mouse lung, mouse hippocampus

GART Antibody Western Blot, validation (1:500 dilution) in HepG2, mouse liver, mouse lung, mouse hippocampus (Cat no:13659-1-AP)