Anticorps Monoclonal anti-GAS2L1

GAS2L1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2C6D5

N° de cat : 68051-1-PBS

Synonymes

GAR22, GAS2 like protein 1, GAS2L1, growth arrest 2 like 1

---

Galerie de données de validation

• 

  WB analysis using 68051-1-Ig (same clone as 68051-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 68051-1-Ig (GAS2L1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68051-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 68051-1-Ig (same clone as 68051-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 68051-1-Ig (GAS2L1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68051-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 68051-1-Ig (same clone as 68051-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68051-1-Ig (GAS2L1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68051-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 68051-1-Ig (same clone as 68051-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68051-1-Ig (GAS2L1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68051-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

68051-1-PBS cible GAS2L1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GAS2L1 Protéine recombinante Ag31232
• Nom complet: growth arrest-specific 2 like 1
• Masse moléculaire calculée: 73 kDa
• Poids moléculaire observé: 73 kDa
• Numéro d’acquisition GenBank: BC011047
• Symbole du gène: GAS2L1
• Identification du gène (NCBI): 10634
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GAS2L1(growth arrest‐specific 2‐like 1) is a component of the cytoskeleton that mediates the interaction between microtubules and microfilament (PMID: 34555082). GAS2L1 contains an N‐terminal actin‐binding calponin homology (CH) domain, a MT lattice‐binding Gas2‐related (GAR) domain and a C‐terminal SxIP motif, which mediates the interaction with MT plus ends via EB proteins. GAS2L1 regulates microtubule dynamics and stability by interacting with microtubule plus-end tracking proteins, such as MAPRE1, to regulate microtubule growth along actin stress fibers (PubMed:24706950).

{{ptg:RelatedPrimaryAntibodies}}