Anticorps Polyclonal de lapin anti-GATA1

• Phare
• Validé par KD/KO

GATA1 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 10917-2-AP

Synonymes

ERYF1, Erythroid transcription factor, GATA 1, GATA binding factor 1, GATA1, GF 1, GF1, NF E1 DNA binding protein, NFE1, XLTT

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Galerie de données de validation

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  WB analysis using 10917-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10917-2-AP (GATA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 10917-2-AP

  WB result of GATA1 antibody (10917-2-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GATA1 transfected K-562 cells.

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  WB analysis of Raji using 10917-2-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10917-2-AP (GATA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 10917-2-AP

  IP Result of anti-GATA1 (IP:10917-2-AP, 4ug; Detection:10917-2-AP 1:500) with K-562 cells lysate 4000ug.

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  IHC staining of mouse testis using 10917-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10917-2-AP (GATA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 10917-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10917-2-AP (GATA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules K-562, cellules NIH/3T3, cellules Raji
• Résultats positifs en IP: cellules K-562
• Résultats positifs en IHC: tissu testiculaire de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 4 publications below

Informations sur le produit

10917-2-AP cible GATA1 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GATA1 Protéine recombinante Ag1350
• Nom complet: GATA binding protein 1 (globin transcription factor 1)
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 50-55 kDa, 40-45 kDa
• Numéro d’acquisition GenBank: BC009797
• Symbole du gène: GATA1
• Identification du gène (NCBI): 2623
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GATA1 is first identified as a member of the GATA transcription factor family, whose members bind the consensus (WGATAR) binding motif [PMID:22937757]. GATA1, a zinc finger DNA-binding transcription factor, plays a critical role in the normal development of hematopoietic cell lineages. The protein contains an N-terminal region that confers transcriptional activity and a C-terminal domain that mediates binding to DNA and other factors [PMID: 8524811]. GATA-1 is also implicated in regulating the expression of the erythroid and megakaryocytic-specific genes [PMID:22937757]. GATA1 exists two isoform with the molecular weight 50-55 kDa and 40-45 kDa (PMID: 31189107 ).

Publications

Species	Application	Title
human	WB,IHC	Oncogene; microRNA-23a, -27a and -24 synergistically regulate JAK1/Stat3 cascade and serve as novel therapeutic targets in human acute erythroid leukemia.; Authors - R Su
human	WB	Front Immunol; FLI1 Induces Megakaryopoiesis Gene Expression Through WAS/WIP-Dependent and Independent Mechanisms; Implications for Wiskott-Aldrich Syndrome.; Authors - Chunlin Wang
mouse	IHC	Genes (Basel); Proof of Gene Doping in a Mouse Model with a Human Erythropoietin Gene Transferred Using an Adenoviral Vector.; Authors - Takehito Sugasawa
human	WB,IHC	Oncotarget; The transcription factor GATA1 and the histone methyltransferase SET7 interact to promote VEGF-mediated angiogenesis and tumor growth and predict clinical outcome of breast cancer.; Authors - Yanan Zhang
human	WB	Biomed Pharmacother; Xanthotoxin, a novel inducer of platelet formation, promotes thrombocytopoiesis via IL-1R1 and MEK/ERK signaling; Authors - Jia Lai
human	WB,IHC	Mol Cell Biochem; GATA1 promotes colorectal cancer cell proliferation, migration and invasion via activating AKT signaling pathway.; Authors - Junhui Yu ; KD Validated