Anticorps Monoclonal anti-GATA1

• Phare
• Validé par KD/KO

GATA1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(9)

CloneNo.

5E2A8

N° de cat : 60011-1-Ig

Synonymes

5E2A8, ERYF1, GATA-1, GATA-binding factor 1, GF1

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Galerie de données de validation

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  WB analysis using 60011-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60011-1-Ig (GATA1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 60011-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 60011-1-Ig (GATA1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 60011-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 60011-1-Ig (GATA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat testis using 60011-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 60011-1-Ig (GATA1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 60011-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 60011-1-Ig (GATA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 60011-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 60011-1-Ig (GATA1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 60011-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GATA1 antibody (60011-1-Ig, Clone: 5E2A8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules Jurkat, cellules K-562, cellules Raji
• Résultats positifs en IHC: tissu testiculaire de souris, tissu testiculaire de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

60011-1-Ig cible GATA1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GATA1 Protéine recombinante Ag1350
• Nom complet: GATA binding protein 1 (globin transcription factor 1)
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: BC009797
• Symbole du gène: GATA1
• Identification du gène (NCBI): 2623
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GATA1 is first identified as a member of the GATA transcription factor family, whose members bind the consensus (WGATAR) binding motif [PMID:22937757]. GATA1, a zinc finger DNA-binding transcription factor, plays a critical role in the normal development of hematopoietic cell lineages. The protein contains an N-terminal region that confers transcriptional activity and a C-terminal domain that mediates binding to DNA and other factors [PMID: 8524811]. GATA-1 is also implicated in regulating the expression of the erythroid and megakaryocytic-specific genes [PMID:22937757].

Publications

Species	Application	Title
human	IP	Blood; PML4 facilitates erythroid differentiation by enhancing the transcriptional activity of GATA-1.; Authors - Wu Jie J
human	IF	Development; The PAX-SIX-EYA-DACH network modulates GATA-FOG function in fly hematopoiesis and human erythropoiesis.; Authors - T Michael Creed ; KO Validated
human	WB	Biochem J; ZNF16 (HZF1) promotes erythropoiesis and megakaryocytopoiesis via its regulation on c-KIT gene.; Authors - Chen Jing J
human	WB	J Cell Mol Med; 1,25(OH)2 D3 blocks IFNβ production through regulating STING in epithelial layer of oral lichen planus.; Authors - Xuejun Ge
human	WB	Cell Death Discov; JAC1 suppresses proliferation of breast cancer through the JWA/p38/SMURF1/HER2 signaling.; Authors - Yanlin Ren
human	WB	Cell Death Dis; GATA3 recruits UTX for gene transcriptional activation to suppress metastasis of breast cancer.; Authors - Wenqian Yu

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