Anticorps Monoclonal anti-GATA1

• Phare
• Validé par KD/KO

GATA1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

5E2A8

N° de cat : 60011-1-PBS

Synonymes

5E2A8, ERYF1, GATA-1, GATA-binding factor 1, GF1

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Galerie de données de validation

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  WB analysis using 60011-1-Ig (same clone as 60011-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60011-1-Ig (GATA1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60011-1-PBS in a different storage buffer formulation.

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  WB analysis of Raji using 60011-1-Ig (same clone as 60011-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 60011-1-Ig (GATA1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60011-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 60011-1-Ig (same clone as 60011-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 60011-1-Ig (GATA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60011-1-PBS in a different storage buffer formulation.

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  IHC staining of rat testis using 60011-1-Ig (same clone as 60011-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 60011-1-Ig (GATA1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60011-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat testis using 60011-1-Ig (same clone as 60011-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 60011-1-Ig (GATA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60011-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 60011-1-Ig (same clone as 60011-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 60011-1-Ig (GATA1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60011-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 60011-1-Ig (same clone as 60011-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GATA1 antibody (60011-1-Ig, Clone: 5E2A8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60011-1-PBS in a different storage buffer formulation.

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Informations sur le produit

60011-1-PBS cible GATA1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GATA1 Protéine recombinante Ag1350
• Nom complet: GATA binding protein 1 (globin transcription factor 1)
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: BC009797
• Symbole du gène: GATA1
• Identification du gène (NCBI): 2623
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GATA1 is first identified as a member of the GATA transcription factor family, whose members bind the consensus (WGATAR) binding motif [PMID:22937757]. GATA1, a zinc finger DNA-binding transcription factor, plays a critical role in the normal development of hematopoietic cell lineages. The protein contains an N-terminal region that confers transcriptional activity and a C-terminal domain that mediates binding to DNA and other factors [PMID: 8524811]. GATA-1 is also implicated in regulating the expression of the erythroid and megakaryocytic-specific genes [PMID:22937757].

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