Anticorps Polyclonal de lapin anti-GATA3

• Phare
• Validé par KD/KO

GATA3 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ChIP, ELISA

Conjugaison

Non conjugué

Publications

29

N° de cat : 10417-1-AP

Synonymes

GATA binding factor 3, GATA binding protein 3, GATA3, HDR

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Galerie de données de validation

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  WB analysis of MCF-7 using 10417-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10417-1-AP (GATA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10417-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10417-1-AP (GATA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10417-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10417-1-AP (GATA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 10417-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10417-1-AP (GATA3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10417-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10417-1-AP (GATA3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules HeLa
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 19 publications below
• ChIP: See 1 publications below

Informations sur le produit

10417-1-AP cible GATA3 dans les applications de WB, IHC, ChIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GATA3 Protéine recombinante Ag0174
• Nom complet: GATA binding protein 3
• Masse moléculaire calculée: 443 aa, 48 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: BC003070
• Symbole du gène: GATA3
• Identification du gène (NCBI): 2625
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GATA3 is a transcriptional factor that binds to the enhancer elements of all 4 T-cell antigen receptor genes. GATA3 is highly expressed in naive, freshly activated cells and Th2 lineage cells and thought to be necessary and sufficient for Th2 cytokine gene expression. GATA3 and TBET interfer with each other to regulate gene expression. This is a rabbit polyclonal antibody raised against part of C-terminus of human GATA3.

Publications

Species	Application	Title
human		Cancer Cell; Lunatic fringe deficiency cooperates with the Met/Caveolin gene amplicon to induce basal-like breast cancer.; Authors - Keli Xu
		Cancer Res; Cooperation between Pik3ca and p53 mutations in mouse mammary tumor formation.; Authors - Adams Jessica R JR
	WB	Oxid Med Cell Longev; Androgen Plays a Potential Novel Hormonal Therapeutic Role in Th17 Cells Predominant Neutrophilic Severe Asthma by Attenuating BECs Regulated Th17 Cells Differentiation via MBD2 Expression; Authors - Binaya Wasti
human	WB	Cell Death Dis; Calreticulin promotes EGF-induced EMT in pancreatic cancer cells via Integrin/EGFR-ERK/MAPK signaling pathway.; Authors - Weiwei Sheng
human		Development; The inhibitor of histone acetyltransferases Nir regulates epidermis development.; Authors - Delphine Duteil
human	WB	Int J Cardiol; Endothelial microparticles exert differential effects on functions of Th1 in patients with acute coronary syndrome.; Authors - Lu Yongguang Y

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