Anticorps Polyclonal de lapin anti-GATA3

• Phare
• Validé par KD/KO

GATA3 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 22343-1-AP

Synonymes

GATA binding factor 3, GATA-binding factor 3, Trans-acting T-cell-specific transcription factor GATA-3

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Galerie de données de validation

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  WB analysis of MCF-7 using 22343-1-AP

  WB result of GATA3 antibody (22343-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GATA3 transfected MCF-7 cells.

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  WB analysis of mouse thymus using 22343-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 22343-1-AP (GATA3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 22343-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22343-1-AP (GATA3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 22343-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using GATA3 antibody (22343-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules T-47D, tissu de thymus de souris
• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

22343-1-AP cible GATA3 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GATA3 Protéine recombinante Ag17833
• Nom complet: GATA binding protein 3
• Masse moléculaire calculée: 443 aa, 48 kDa
• Poids moléculaire observé: 37 kDa and 48 kDa
• Numéro d’acquisition GenBank: BC006793
• Symbole du gène: GATA3
• Identification du gène (NCBI): 2625
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Trans-acting T-cell-specific transcription factor, also known as GATA-3, belongs to the zinc-finger transcription factor GATA family which drives the differentiation of T helper (Th) 2 cells. GATA factors are expressed in a tissue-specific manner and GATA3 is present in both hematopoietic (e.g., T cells) and non-hematopoietic tissues, including the kidney, central nervous system, skin and mammary gland (PMID: 25148023). GATA-3 plays a central role in Th2 responses both in vitro and in vivo (PMID: 16467870). The full length GATA3 protein contains 444 aa corresponding to the molecular weights of 48,0 kDa, and the truncated protein was deteced at approximately 37 kDa (PMID: 24758297).

Publications

Species	Application	Title
mouse	WB	Life Sci; Berberine regulates macrophage polarization through IL-4-STAT6 signaling pathway in Helicobacter pylori-induced chronic atrophic gastritis.; Authors - Tao Yang

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