Anticorps Polyclonal de lapin anti-GATA3

• Phare
• Validé par KD/KO

GATA3 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 22343-1-PBS

Synonymes

GATA binding factor 3, GATA-binding factor 3, Trans-acting T-cell-specific transcription factor GATA-3

---

Galerie de données de validation

• 

  WB analysis of MCF-7 using 22343-1-AP (same clone as 22343-1-PBS)

  WB result of GATA3 antibody (22343-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GATA3 transfected MCF-7 cells. This data was developed using the same antibody clone with 22343-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse thymus using 22343-1-AP (same clone as 22343-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 22343-1-AP (GATA3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22343-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 22343-1-AP (same clone as 22343-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22343-1-AP (GATA3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22343-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 22343-1-AP (same clone as 22343-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using GATA3 antibody (22343-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 22343-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

22343-1-PBS cible GATA3 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GATA3 Protéine recombinante Ag17833
• Nom complet: GATA binding protein 3
• Masse moléculaire calculée: 443 aa, 48 kDa
• Poids moléculaire observé: 37 kDa and 48 kDa
• Numéro d’acquisition GenBank: BC006793
• Symbole du gène: GATA3
• Identification du gène (NCBI): 2625
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Trans-acting T-cell-specific transcription factor, also known as GATA-3, belongs to the zinc-finger transcription factor GATA family which drives the differentiation of T helper (Th) 2 cells. GATA factors are expressed in a tissue-specific manner and GATA3 is present in both hematopoietic (e.g., T cells) and non-hematopoietic tissues, including the kidney, central nervous system, skin and mammary gland (PMID: 25148023). GATA-3 plays a central role in Th2 responses both in vitro and in vivo (PMID: 16467870). The full length GATA3 protein contains 444 aa corresponding to the molecular weights of 48,0 kDa, and the truncated protein was deteced at approximately 37 kDa (PMID: 24758297).

{{ptg:RelatedPrimaryAntibodies}}